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Available ordering format
Lyophilized
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Immunogen
KLH-conjugated synthetic peptide derived from known sequences of RPN6 including Arabidopsis thaliana RPN6 sequence, UniProt: Q9LP45, TAIR: AT1G29150
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Affinity purified serum in PBS pH 7.4
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How to reconstitute
For reconstitution add 50 µl of sterile water.
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Storage condition
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applications
western blot (WB)
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Connected products
AS15 2832 | anti-RPN6 | 26S proteasome non-ATPase regulatory subunit 9 (other species), rabbit antibodies AS08 308 | anti-SUMO1 | Small ubiquitin-like modifier protein 1, rabbit antibodies AS08 349 | anti-SUMO3 | Small ubiquitin-like modifier protein 3, rabbit antibodies AS08 307 | anti-UBQ11 | Ubiquitin, rabbit antibodies AS08 307A | anti-UBQ11 | Ubiquitin, affinity purified rabbit antibody AS10 307S | UBQ11 | Ubiquitin protein (positive control) AS01 019 | anti-S5a | human 26S proteasome subunit, rabbit antibodyAS01 012 | anti-DS5a | Drosophila 26S proteasome subunit Rpn10, rabbit antibodySecondary antibodies
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Recommended dilutions for use
1:1000 (WB)
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Molecular weight expected аpparent
40 kDa
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Verified reactivity
Arabidopsis thaliana
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Possible reactivity
Glycine soja , Gossypium arboreum, Medicago truncatula, Ornithogalum saundersiae, Oryza sativa, Populus trichocarpa, Ricinus communis, Solanum chacoense, Theobroma caco, Triticum urartu, Zea mays, Zostera marina
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No reactivity
to be determined
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Supplementary information
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References
To be added when available, antibody released in May 2016.
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Scientific context
Proteasome-dependent degradation serves an essential role in the removal of a wide variety of key nuclear and cytosolic proteins. Substrates are targeted for proteolysis by the ubiquitin pathway before being degraded by the 26 S proteasome. RPN6 associates with an ATPase subunit of the 19S proteasome regulatory complex, AtS6A. Alternative names: 26S proteasome non-ATPase regulatory subunit 11 homolog, 19S proteosome subunit 9, AtS9 26S proteasome regulatory subunit RPN6, AtRPN6, 26S proteasome regulatory subunit S9 homolog.
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Notes
None
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Protein number
Q9LP45
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TAIR number
AT1G29150
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