-
Description
RPMI-1640, w/ 3.05mM L-Glutamine, 10mM HEPES buffer and 2gms per litre Sodium bicarbonate, RPMI were developed for the culture of human normal and neoplastic cells in vitro. It was specifically designed to support the growth of human lymphoblastoid cells in suspension culture. Now the medium is extensively used for a wide range of anchorage dependant cell lines. It is also known to support growth of cells in the absence of serum.
-
-
Shelf life
Use before expiry date given on the product label.
-
Storage
Store at 2-8°C away from bright light
-
Appearance
Orangish red colored, clear solution
-
PH
NA
-
Osmolality in mOsm Kg H2O
NA
-
Sterility
NA
-
Cultural Response
NA
-
Endotoxin Content
NA
-
Uses
Laboratory chemicals, Manufacture of substances
-
Personal Protection
Use personnel protective equipment. Wear disposable gloves, dust mask and eye protection. Avoid dust formation.
-
Toxicological Information
NA
-
Technical Data
Contact us to request a Technical Data
-
-
Ordering
To order RPMI-1640 w/ 3.05mM L-Glutamine, 10mM HEPES buffer and 2gms per litre Sodium bicarbonate, please use the Cat. Nr.AL214A-40X500ML and submit your purchase order by email or by fax. A discount is available for larger or bulk quantities, please contact us for more information
-
Tips
Our specialists recommend you to follow carefully the pre-registered instructions for RPMI-1640 w/ 3.05mM L-Glutamine, 10mM HEPES buffer and 2gms per litre Sodium bicarbonate
-
Additional description
Buffering solutions are useful to keep the pH range sable when using this reagent of Himedia. The Liter or liter is a metric system unit of volume equal to 1 cubic decimeter (dm3), 1,000 cubic centimeters (cm3) or 1/1,000 cubic meter. A cubic decimeter (or liter) occupies a volume of 10×10×10 centimeters (see figure) and is thus equal to one-thousandth of a cubic meter. Water baths of various volumes are available for lab research.
-
Buffer
25mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) pH7 solution buffers are a media with a zwitterion organic chemical buffering agent; one of the twenty Good's buffers. HEPES DMEM medium is widely used in cell culture, largely because it is better at maintaining physiological pH 7 tot 7,5 despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. The dissociation of water decreases with falling temperature, but the dissociation constants (pK) of many other buffers do not change much with temperature. HEPES, 25 mM in RPMI and DMEM is like water in that its dissociation decreases as the temperature decreases. This makes HEPES RPMI 25 milli-molar media with or without L-glutamine a more effective buffering agent for maintaining enzyme structure and function at low temperatures.