RPB1, RNA polymerase II subunit B1

• Catalog number
  AS11 1804
• Price
  Please ask
• Size
  50 µg

• Available ordering format
  Lyophilized
• Immunogen
  KLH-conjugated synthetic peptide derived from known higher plant polymerase II sequences, including Arabidopsis thaliana P18616, At4g35800
• Raised in
  Rabbit
• Clonality
  Polyclonal
• Clone
  Polyclonal
• Purification
  Affinity purified serum
• How to reconstitute
  For reconstitution add 50 µl of sterile water.
• Storage condition
  store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
• Verified applications
  immunoprecipitation (IP)
• Connected products
  AS10 710 | anti-H3 | histone 3, rabbit antibody collection of antibodies to DN/RNA/cell cyclePlant and algal protein extraction buffer Secondary antibodies
• Recommended dilutions for use
  1,2 or 5 µg (IP)
• Molecular weight expected аpparent
  204 kDa
• Verified reactivity
  Arabidopsis thaliana
• Possible reactivity
  Amygdalus persica, Asparagus officinalis, Brassica rapa, Capsella rubella, Glycine max, Hordeum vulgare, Solanum lycopersicum, Oryza glaberrima, Panicum italicum, Persea americana, Pisum sativum, Ricinus communis, Solanum tuberosum, Sorghum vulgare, Vitis vinifera
• No reactivity
  no confirmed exceptions from predicted reactivity are currently known
• Supplementary information
  Contact Gentaur support center at [email protected]
• References
  Dolata et al. (2015). NTR1 is required for transcription elongation checkpoints at alternative exons in Arabidopsis. EMBO J. 2015 Jan 7. pii: e201489478.
• Scientific context
  DNA-directed RNA polymerase II subunit 1 (EC=2.7.7.6) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. This enzyme is the central component of the basal RNA polymerase II transcription machinery. Synonymes:DNA-directed RNA polymerase III largest subunit,DNA-directed RNA polymerase II subunit RPB1.
• Notes
  Purified IgG was lyophilized from PBS pH 7.4 + 0.02 % sodium azide. Therefore only distilled water is required for its re-constitution.
• Protein number
  P18616
• TAIR number
  AT4G35800
• Hyperlink

• Gene target
  RPB1   RNA   polymerase   subunit  
• Gene symbol
  POLR2A
• Short name
  RPB1, RNA polymerase II subunit B1
• Technique
  RNAs are to store at -70 C
• Alternative name
  RPB1, RNA polymerizing enzyme II functionnal sequence B1

Gene info

• Identity
  HGNC:9187
• Gene
  POLR2A
• Long gene name
  RNA polymerase II subunit A
• Synonyms gene
  • POLR2
• Synonyms gene name
  • polymerase (RNA) II (DNA directed) polypeptide A, 220kDa
• Synonyms
  • POLRA
  • RPB1
• Synonyms name
  • DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit
  • RNA polymerase II subunit B1
• Locus
  17p13.1
• Discovery year
  1986-01-01
• Entrez gene record
  5430
• RefSeq identity
  • NM_000937
• Classification
  • RNA polymerase subunits
• VEGA ID
  OTTHUMG00000177594

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