RPA2(Thr21) Antibody, ALEXA FLUOR 350

• Catalog number
  bs-5693R-A350
• Price
  Please ask
• Size
  100ul

• Long name
  RPA2(Thr21) Polyclonal Antibody, ALEXA FLUOR 350 Conjugated
• Also known as
  Anti-RPA2Thr21 PAb ALEXA FLUOR 350
• Category
  Conjugated Primary Antibodies
• Conjugated with
  ALEXA FLUOR® 350
• Host Organism
  Rabbit (Oryctolagus cuniculus)
• Target Antigen
  RPA2Thr21
• Specificity
  This is a highly specific antibody against RPA2Thr21.
• Modification
  Phosphorylation
• Modification Site
  Thr21
• Clonality
  Polyclonal
• Clone
  Polyclonal antibody
• Concentration
  1ug per 1ul
• Immunogen range
  5-40/270
• Subcellular location
  Nucleus
• Source
  KLH conjugated synthetic phosphopeptide derived from human RPA2 around the phosphorylation site of Thr21
• Gene ID Number
  6118
• Swiss Prot
  P15927
• Tested applications
  IF(IHC-P)
• Recommended dilutions
  IF(IHC-P)(1:50-200)
• Crossreactivity
  Human, Mouse, Rat
• Cross reactive species details
  Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
• Background of the antigen
  As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Plays also a role in base excision repair (BER) probably through interaction with UNG. Through RFWD3 may activate CHEK1 and play a role in replication checkpoint control. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance.
• Purification
  Purified by Protein A.
• Storage conditions
  Store this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.
• Excitation emission
  343nm/442nm
• Synonyms
  REPA2; RPA32; RP-A p32; RP-A p34; Replication protein A 32 kDa subunit; Replication factor A protein 2; RF-A protein 2; Replication protein A 34 kDa subunit; RPA2; RPA34
• Properties
  For facs or microscopy Alexa 1 conjugate. Alexa Fluor 350 conjugates can be used in multi-color flow cytometry with FACS's equipped with a second red laser or red diode. If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• Conjugation
  Alexa Fluor, ALEXA FLUOR 350
• French translation
  anticorps

• Gene target
  RPA2   Thr21  
• Gene symbol
  RPA2, POLR1B
• Short name
  Anti-RPA2Thr21
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Immunoglobulin G (IgG)
• Label
  ALEXA FLUOR 350
• Alternative name
  RPA2(Thr21) Polyclonal Antibody, ALEXA FLUOR 350
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:10290
• Gene
  RPA2
• Long gene name
  replication protein A2
• Synonyms gene name
  • replication protein A2 (32kD)
  • replication protein A2, 32kDa
• GenBank acession
  • BC021257
• Locus
  1p35.3
• Discovery year
  1993-12-14
• Entrez gene record
  6118
• Pubmed identfication
  • 8454588
• RefSeq identity
  • NM_002946
• Classification
  • Nucleotide excision repair
• VEGA ID
  OTTHUMG00000003915

Gene info

• Identity
  HGNC:20454
• Gene
  POLR1B
• Long gene name
  RNA polymerase I subunit B
• Synonyms gene name
  • polymerase (RNA) I polypeptide B, 128kDa
• Synonyms
  • Rpo1-2
  • FLJ21921
  • FLJ10816
  • RPA2
  • RPA135
• GenBank acession
  • AK001678
• Locus
  2q14.1
• Discovery year
  2003-04-01
• Entrez gene record
  84172
• RefSeq identity
  • NM_019014
• Classification
  • RNA polymerase subunits
• VEGA ID
  OTTHUMG00000131314

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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