RIPK2, Active

• Catalog number
  7747-5
• Price
  Please ask
• Size
  5 ug

• Synonyms
  RIPK, receptor-interacting serine-threonine kinase 2
• Alternative_names
  RIPK, receptor-interacting serine-threonine kinase 2, CARD-containing interleukin-1 beta-converting enzyme-associated kinase, Short name=CARD-containing IL-1 beta ICE-kinase, RIP-like-interacting CLARP kinase, Receptor-interacting protein 2, Tyrosine-protein kinase RIPK2
• Description
  A potent activator of NF-κB and inducer of apoptosis
• Recombinant
  Yes
• Source
  Baculovirus (Sf9 insect cells)
• Purity by SDS PAGE
  ≥90%
• Assay
  SDS-PAGE
• Activity Specifications test method
  20 nmol phosphate incorporated into MBP per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 µM ATP (0.83 µCi/assay).
• Molecular Weight
  59.0kDa
• Storage Temp
  -80°C
• Shipping
  dry ice
• Shelf Life
  12 months
• Concentration
  5µg/50 µl
• Appearance
  Liquid
• Physical form description
  Recombinant proteins in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol).
• Background Information
  RIPK2 (RIP2; RICK) is a death domain-containing protein kinase. Inohara identified cDNAs encoding a predicted 540-amino acid protein RICK, which contains an N-terminal serine/threonine kinase catalytic domain and a C-terminal caspase activation and recruitment domain. Inohara also demonstrated that RICK is a novel kinase that may regulate apoptosis induced by the FAS receptor pathway (1). McCarthy found that overexpression of RIP2 signaled both cell death and NF-kappa-B activation (2). Thome reported that RICK specifically interacted with the CARD of ICE/caspase-1, and this interaction correlated with the processing of pro-caspase-1 and the formation of the active caspase-1 p20 (3). Chin generated Ripk2-deficient mice and concluded that RIPK2 is implicated in the innate response to pathogens by NOD and TLR-induced cell signaling and mediates cytokine-induced Ifng production in Th1 and NK cells (4). Also Kobayashi demonstrated that RIPK2 is required for signaling through both TLR and NOD protein family members in the innate immune system as well as for appropriate TCR signaling in the adaptive immune response (5).
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet

• Gene target
  RIPK2   Active  
• Gene symbol
  RIPK2-DT, RIPK2
• Short name
  RIPK2, Active
• Alternative name
  receptor-interacting serine-threonine kinase 2, functionnal
• Alternative to gene target
  receptor-interacting serine-threonine kinase 2, CARD3 and CARDIAK and CCK and GIG30 and RICK and RIP2, RIPK2 and IDBG-27941 and ENSG00000104312 and 8767, transferase activity, Cytoplasm, Ripk2 and IDBG-130745 and ENSMUSG00000041135 and 192656, RIPK2 and IDBG-645358 and ENSBTAG00000015271 and 534407

Gene info

• Identity
  HGNC:55545
• Gene
  RIPK2-DT
• Long gene name
  RIPK2 divergent transcript
• Locus
  8q21.3
• Discovery year
  2021-03-24
• Entrez gene record
  101929709
• RefSeq identity
  • NR_125822
• Classification
  • Divergent transcripts

Gene info

• Identity
  HGNC:10020
• Gene
  RIPK2
• Long gene name
  receptor interacting serine/threonine kinase 2
• Synonyms gene name
  • receptor-interacting serine-threonine kinase 2
• Synonyms
  • RICK
  • RIP2
  • CARDIAK
  • CARD3
• GenBank acession
  • AC004003
• Locus
  8q21.3
• Discovery year
  1999-05-07
• Entrez gene record
  8767
• Pubmed identfication
  • 9575181
  • 9705938
• Classification
  • Caspase recruitment domain containing
• VEGA ID
  OTTHUMG00000163809

Similar products