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Immunogen
A synthesized peptide
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Antibody s target
CD9
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Antibody s full description
Rabbit Anti- CD9 Polyclonal
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Primary research fields
Immunology, Cell Type Markers, CD Markers, Stem Cells, Embryonic Stem Cells, ESC Surface Molecules
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
CD9 antigen Antibody, CD9 antigen p24 Antibody, Leukocyte antigen MIC3 Antibody, Motility-related protein Antibody, Tetraspanin-29 Antibody, Tspan-29 Antibody, TSPAN29 Antibody, BA-2/p24 antigen Antibody, BA2 Antibody, Cell growth inhibiting gene 2 protein Antibody, DRAP 27 Antibody, GIG2 Antibody, Growth inhibiting gene 2 protein Antibody
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Verified applications
WB, IHC, ICC/IF
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Raised in
Rabbit
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Antibody s reactivity
Human, Mouse, Rat
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Antibody s recommended dilutions for use
WB (1:1000), IHC (1:200), ICC/IF (1:200); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Peptide Affinity Purified
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Recommended buffer for storage
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
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Antibody s concentration
See included datasheet or contact our support service
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Antibody s specificity
Detects endogenous levels of total CD9
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:1000 dilution of SPC-1272 was sufficient for detection of CD9 in 10 µg of COLO cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Membrane
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Tissue specificity
Expressed by a variety of hematopoietic and epithelial cells.
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Scientific context
CD9 is a 24 kD type III transmembrane protein also known as tetraspanin, MRP-1 and DRAP-24. It is a member of the tetraspan family (spanning the membrane four times) found on platelets, B cell progenitors, activated lymphocytes, granulocytes, endothelial cells and epithelial cells. CD9 induces adhesion, platelet aggregation, and B cell development. CD9 has been shown to associate with CD63, CD81, CD82, and CD36 and to bind to β1 integrins. It plays a role in cell signaling by mediating interactions with low molecular weight GTP-binding proteins.
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Bibliography
1. Miao W.M., et al. (2001) Blood. 97:1689. 2. Ellerman D.A., et al. (2003) Mol. Biol Cell. 14: 5098-5103. 3. Schlossman S., et al. (1995) Leucocyte Typing V. Oxford University Press. New York.
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Released date
6/Sep/2016
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NCBI number
NP_001760.1
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Gene number
928
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Protein number
P21926
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure link
No Data Available
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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Gene target
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Gene symbol
CD9
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Short name
Rabbit Anti- CD9 Polyclonal: ATTO 390
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Technique
Polyclonal, Rabbit, anti, antibody to, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Host
Rabbit, Rabbits
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Isotype
IgG
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Label
ATTO 390
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Alternative name
production species: rabbit antibody to- CD9 molecule polyclonal: ATTO 390
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Alternative technique
polyclonals, rabbit-anti, antibodies
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Alternative to gene target
CD9 molecule, BTCC-1 and DRAP-27 and MIC3 and MRP-1 and TSPAN-29 and TSPAN29, CD9 and IDBG-13612 and ENSG00000010278 and 928, protein binding, Extracellular, Cd9 and IDBG-189938 and ENSMUSG00000030342 and 12527, CD9 and IDBG-640522 and ENSBTAG00000014764 and 280746
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