Recombinant Rat GPT1 / GPT Protein (His tag)

• Catalog number
  PKSR030415-10µg
• Price
  Please ask
• Size
  10µg

• Synonym
  Gpt1;Gpt;ALT1
• Activity
  NA
• Sequence
  Met 1-Ser 496
• Fusion tag
  C-His
• Accession
  NP_112301.1
• ExpressedHost
  Baculovirus-Insect Cells
• Shipping
  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
• Purity
  > 95 % as determined by SDS-PAGE
• Endotoxin
  < 1.0 EU per μg of the protein as determined by the LAL method
• Stability and Storage
  Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Mol Mass
  55 kDa
• AP Mol Mass
  48 KDa
• Formulation
  Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 8.0, 10% glycerol, 0.5mM TCEP
• Background
  Alanine aminotransferase (ALT), also known as glutamate pyruvate transaminase (GPT), is a pyridoxal enzyme which belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family, Alanine aminotransferase subfamily. Gpt / Gpt1 / ALT catalyses the reversible interconversion of L-alanine and 2-oxoglutalate to pyruvate and L-glutamate, and plays a key role in the intermediary metabolism of glucose and amino acids. Gpt / Gpt1 / ALT is expressed in Liver, kidney, heart, and skeletal muscles and it expresses at moderate levels in the adipose tissue. As a key enzyme for gluconeogenesis, Gpt is a widely-used serum marker for liver injury. Two ALT isoenzymes have been identified, ALT1 and ALT2 (GPT1 and GPT2), which are encoded by separate genes and share significant sequence homology, but differ in their expression patterns. GPT1/GPT is widely distributed and mainly expressed in intestine, liver, fat tissues, colon, muscle, and heart, in the order of high to low expression level. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and steatosis.
• Technical data
• Conjugation
  histidine
• About
  Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
• Latin name
  Rattus norvegicus
• Source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  GPT1   GPT   Protein   His   tag  
• Gene symbol
  GPT, DPAGT1
• Short name
  Recombinant GPT1 / GPT Protein (His tag)
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
• Host
  Rat
• Label
  His
• Species
  Rat, Rats
• Alternative name
  Rec. Rat GPT1 / GPT Protein (histidine detection labelled)
• Alternative technique
  rec

Gene info

• Identity
  HGNC:4552
• Gene
  GPT
• Long gene name
  glutamic--pyruvic transaminase
• Synonyms gene name
  • glutamic-pyruvate transaminase (alanine aminotransferase)
• Synonyms
  • ALT1
  • GPT1
• Synonyms name
  • alanine aminotransferase
  • alanine transaminase
• Locus
  8q24.3
• Discovery year
  2001-06-22
• Entrez gene record
  2875
• VEGA ID
  OTTHUMG00000165176

Gene info

• Identity
  HGNC:2995
• Gene
  DPAGT1
• Long gene name
  dolichyl-phosphate N-acetylglucosaminephosphotransferase 1
• Synonyms gene
  • DPAGT2
  • DPAGT
• Synonyms gene name
  • dolichyl-phosphate (UDP-N-acetylglucosamine) N-acetylglucosaminephosphotransferase 1 (GlcNAc-1-P transferase)
• Synonyms
  • GPT
  • D11S366
  • DGPT
  • ALG7
  • CDG-Ij
• Synonyms name
  • GlcNAc-1-P transferase 1
  • UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase 1
• GenBank acession
  • Z82022
• Locus
  11q23.3
• Discovery year
  1993-12-13
• Entrez gene record
  1798
• Pubmed identfication
  • 8244387
• RefSeq identity
  • NM_001382
• VEGA ID
  OTTHUMG00000153533
• Locus Specific Databases
  • Congenital Disorders of Glycosylation pages

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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