Recombinant Mouse R-Spondin 1 / RSPO1 Protein (His tag)

• Catalog number
  PKSM040742-20µg
• Price
  Please ask
• Size
  20µg

• Synonym
  R-spondin;Rspondin
• Activity
  Measured by its ability to induce activation of β-catenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells. The ED50 for this effect is typically 50-200 ng/ml in the presence of 50 ng/ml recombinant mouse Wnt3a.
• Sequence
  Met 1-Gln 265
• Fusion tag
  C-His
• Accession
  NP_619624.2
• Expressed Host
  CHO Stable Cells
• Shipping
  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
• Purity
  > 95 % as determined by SDS-PAGE
• Endotoxin
  < 1.0 EU per μg of the protein as determined by the LAL method
• Stability and Storage
  Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Mol Mass
  28.5 kDa
• AP Mol Mass
  44 kDa
• Formulation
  Lyophilized from sterile PBS, pH 7.4
• Background
  RSPO1 gene is a member of the R-spondin family. It encodes RSPO1 which is known as a secreted activator protein with two cystein-rich, furin-like domains and one thrombospondin type 1 domain. In mice, RSPO1 induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects. This protein is an activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. RSPO1 acts both in the canonical Wnt/beta-catenin-dependent pathway and in non-canonical Wnt signaling pathway, probably by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. It also acts as a ligand for frizzled FZD8 and LRP6.
• Technical data
• Conjugation
  histidine
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus
• Source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  R-Spondin   RSPO1   Protein   His   tag  
• Gene symbol
  RSPO1
• Short name
  Recombinant Mouse R-Spondin 1 / RSPO1 Protein (His tag)
• Technique
  Recombinant, Mouse, mouses, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
• Host
  mouse
• Label
  His
• Species
  Mouse, Mouses
• Alternative name
  Rec. Mouse R-Spondin 1 / RSPO1 Protein (histidine detection labelled)
• Alternative technique
  rec, murine

Gene info

• Identity
  HGNC:21679
• Gene
  RSPO1
• Long gene name
  R-spondin 1
• Synonyms gene name
  • R-spondin homolog (Xenopus laevis)
• Synonyms
  • FLJ40906
  • RSPONDIN
• GenBank acession
  • AK098225
• Locus
  1p34.3
• Discovery year
  2005-08-08
• Entrez gene record
  284654
• Pubmed identfication
  • 17041600
• RefSeq identity
  • NM_173640
• Classification
  • R-spondin family
• VEGA ID
  OTTHUMG00000004321

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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