Recombinant Mouse PARP-1 / PARP Protein (His tag)

• Catalog number
  PKSM040501-20µg
• Price
  Please ask
• Size
  20µg

• Synonym
  5830444G22Rik;Adprp;Adprt1;AI893648;ARTD1;C80510;PARP;parp-1;PPOL;sPARP-1
• Activity
  1. Measured by its binding ability in a functional ELISA. 2. Immobilized mouse PARP1 at 10 μg/mL (100 μl/well) can bind  biotinylated human HSP70, The EC50 of biotinylated human HSP70 is 0.021 μg/mL.
• Sequence
  Met 1-Trp 1014
• Fusion tag
  N-His
• Accession
  NP_031441.2
• Expressed Host
  Baculovirus-Insect Cells
• Shipping
  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
• Purity
  > 85 % as determined by SDS-PAGE
• Endotoxin
  < 1.0 EU per μg of the protein as determined by the LAL method
• Stability and Storage
  Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Mol Mass
  115 kDa
• AP Mol Mass
  75 kDa
• Formulation
  Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 8.0, 10% gly, 0.1mM TCEP
• Background
  Poly (ADP-ribose) polymerase 1(PRAP1), also known as NAD(+) ADP-ribosyltransferase 1(ADPRT), is a chromatin-associated enzyme which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The ADP-D-ribosyl group of NAD+ is transferred to an acceptor carboxyl group on a histone or the enzyme itself, and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 20-30 units. The poly(ADP-ribosyl)ation modification is critical for a wide range of processes, including DNA repair, regulation of chromosome structure, transcriptional regulation, mitosis and apoptosis. PARP1 is demonstrateed to mediate the poly(ADP-ribose) ation of APLF (aprataxin PNK-like factor) and CHFR (checkpoint protein with FHA and RING domains), two representative proteins involved in the DNA damage response and checkpoint regulation. Further, It has been suggested that DNA-dependent protein kinase (DNA-PK), another component of DNA repair, suppresses PARP activity, probably through direct binding and/or sequestration of DNA-ends which serve as an important stimulator for both enzymes. PARP1 inhibitors is thus proposed as a targeted cancer therapy for recombination deficient cancers, such as BRCA2 tumors.Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy
• Technical data
• Conjugation
  histidine
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus
• Source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  PARP-1   PARP   Protein   His   tag  
• Gene symbol
  PARP1, TIPARP, TNKS2, PARP12, TNKS, IGHVII-1-1, MIR1-1, TRUND-NNN8-1, TRUND-NNN7-1, IGKV1OR2-1
• Short name
  Recombinant Mouse PARP-1 / PARP Protein (His tag)
• Technique
  Recombinant, Mouse, mouses, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
• Host
  mouse
• Label
  His
• Species
  Mouse, Mouses
• Alternative name
  Rec. Mouse PARP-1 / PARP Protein (histidine detection labelled)
• Alternative technique
  rec, murine

Gene info

• Identity
  HGNC:270
• Gene
  PARP1
• Long gene name
  poly(ADP-ribose) polymerase 1
• Synonyms gene
  • PPOL
  • ADPRT
• Synonyms gene name
  • ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase)
  • poly (ADP-ribose) polymerase family, member 1
• Synonyms
  • PARP
  • ARTD1
• GenBank acession
  • BC037545
• Locus
  1q42.12
• Discovery year
  1986-01-01
• Entrez gene record
  142
• Pubmed identfication
  • 10964595
• RefSeq identity
  • NM_001618
• Classification
  • Zinc fingers PARP-type
  • Zinc fingers
  • Poly(ADP-ribose) polymerases
• VEGA ID
  OTTHUMG00000037556

Gene info

• Identity
  HGNC:23696
• Gene
  TIPARP
• Long gene name
  TCDD inducible poly(ADP-ribose) polymerase
• Synonyms gene name
  • TCDD-inducible poly(ADP-ribose) polymerase
• Synonyms
  • DKFZP434J214
  • DKFZp686N0351
  • DDF1
  • PARP7
  • PARP-7
  • PARP-1
  • pART14
  • RM1
  • ARTD14
• GenBank acession
  • BX537965
• Locus
  3q25.31
• Discovery year
  2003-12-02
• Entrez gene record
  25976
• Pubmed identfication
  • 12851707
• RefSeq identity
  • NM_015508
• Classification
  • Poly(ADP-ribose) polymerases
• VEGA ID
  OTTHUMG00000158646

Gene info

• Identity
  HGNC:15677
• Gene
  TNKS2
• Long gene name
  tankyrase 2
• Synonyms gene name
  • tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase 2
• Synonyms
  • TNKL
  • TANK2
  • PARP-5b
  • PARP-5c
  • PARP5B
  • PARP5C
  • pART6
  • ARTD6
• Synonyms name
  • TRF1-interacting ankyrin-related ADP-ribose polymerase 2
• GenBank acession
  • AF342982
• Locus
  10q23.32
• Discovery year
  2001-05-10
• Entrez gene record
  80351
• RefSeq identity
  • NM_025235
• Classification
  • Poly(ADP-ribose) polymerases
  • Sterile alpha motif domain containing
  • Ankyrin repeat domain containing
• VEGA ID
  OTTHUMG00000018747

Gene info

• Identity
  HGNC:21919
• Gene
  PARP12
• Long gene name
  poly(ADP-ribose) polymerase family member 12
• Synonyms gene
  • ZC3HDC1
• Synonyms gene name
  • zinc finger CCCH-type domain containing 1
• Synonyms
  • FLJ22693
  • PARP-12
  • ZC3H1
  • ARTD12
• GenBank acession
  • AL136766
• Locus
  7q34
• Discovery year
  2003-12-10
• Entrez gene record
  64761
• Pubmed identfication
  • 11230166
  • 12851707
• RefSeq identity
  • NM_022750
• Classification
  • Poly(ADP-ribose) polymerases
  • Zinc fingers CCCH-type
• VEGA ID
  OTTHUMG00000157315

Gene info

• Identity
  HGNC:11941
• Gene
  TNKS
• Long gene name
  tankyrase
• Synonyms gene name
  • tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase
• Synonyms
  • TIN1
  • TINF1
  • TNKS1
  • PARP-5a
  • PARP5A
  • pART5
  • ARTD5
• Synonyms name
  • TRF1-interacting ankyrin-related ADP-ribose polymerase 1
• GenBank acession
  • AF082556
• Locus
  8p23.1
• Discovery year
  1999-01-08
• Entrez gene record
  8658
• Pubmed identfication
  • 9822378
  • 10198177
• RefSeq identity
  • NM_003747
• Classification
  • Poly(ADP-ribose) polymerases
  • Sterile alpha motif domain containing
  • Ankyrin repeat domain containing
  • MicroRNA protein coding host genes
• VEGA ID
  OTTHUMG00000090481

Gene info

• Identity
  HGNC:5670
• Gene
  IGHVII-1-1
• Long gene name
  immunoglobulin heavy variable (II)-1-1 (pseudogene)
• Synonyms gene name
  • immunoglobulin heavy variable (II)-1-1
  • immunoglobulin heavy variable (II)-1-1 pseudogene
• Synonyms
  • IGHV(II)-1-1
• GenBank acession
  • AB019441
• Locus
  14q32.33
• Discovery year
  2000-04-17
• Entrez gene record
  28377
• RefSeq identity
  • NG_001019
• Classification
  • Immunoglobulin heavy locus at 14q32.33
• VEGA ID
  OTTHUMG00000152325

Gene info

• Identity
  HGNC:31499
• Gene
  MIR1-1
• Long gene name
  microRNA 1-1
• Synonyms gene
  • MIRN1-1
• Synonyms
  • hsa-mir-1-1
• Locus
  20q13.33
• Discovery year
  2004-04-23
• Entrez gene record
  406904
• RefSeq identity
  • NR_029780
• Classification
  • MicroRNA MIR1/206 family

Gene info

• Identity
  HGNC:50820
• Gene
  TRUND-NNN8-1
• Long gene name
  tRNA-undetermined (NNN) 8-1
• Synonyms gene name
  • transfer RNA-undetermined (NNN) 8-1
• GenBank acession
  • HG984097
• Locus
  1
• Discovery year
  2014-06-20
• Entrez gene record
  103482528
• Pubmed identfication
  • 18984615

Gene info

• Identity
  HGNC:50819
• Gene
  TRUND-NNN7-1
• Long gene name
  tRNA-undetermined (NNN) 7-1
• Synonyms gene name
  • transfer RNA-undetermined (NNN) 7-1
• GenBank acession
  • HG984096
• Locus
  1
• Discovery year
  2014-06-20
• Entrez gene record
  103482531
• Pubmed identfication
  • 18984615

Gene info

• Identity
  HGNC:5760
• Gene
  IGKV1OR2-1
• Long gene name
  immunoglobulin kappa variable 1/OR2-1 (pseudogene)
• Synonyms gene
  • IGKVPZ1
  • IGKV1OR-1
• Synonyms gene name
  • immunoglobulin kappa variable 1/OR-1
  • immunoglobulin kappa variable 1/OR-1 pseudogene
  • immunoglobulin kappa variable 1/OR-1 (pseudogene)
• Synonyms
  • IGKV1/OR-1
  • IGKV1/OR2-1
• GenBank acession
  • Z12367
• Locus
  2p11.1
• Discovery year
  2000-04-18
• Entrez gene record
  3531
• Pubmed identfication
  • 2853098
• Classification
  • Immunoglobulin kappa (IGK) orphons
• VEGA ID
  OTTHUMG00000155081

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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