Recombinant Mouse MEP1A Protein (His tag)

• Catalog number
  PKSM040881-10µg
• Price
  Please ask
• Size
  10µg

• Synonym
  AI098089;AW107200;Mep-1;Mep-1a;Mep1
• Activity
  Measured by its ability to cleave a fluorogenic peptide substrate, Mca-YVADAPK(Dnp)-OH, R&D Systems, Catalog # ES007.The specific activity is >400 pmoles/min/μg.(Activation description: The proenzyme needs to be activated by Trypsin for an activated form)
• Sequence
  Met 1-Arg 615
• Fusion tag
  C-His
• Accession
  NP_032611.2
• Expressed Host
  HEK293 Cells
• Shipping
  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
• Purity
  > 95 % as determined by SDS-PAGE
• Endotoxin
  < 1.0 EU per μg of the protein as determined by the LAL method
• Stability and Storage
  Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Mol Mass
  69 kDa
• AP Mol Mass
  80-90 kDa
• Formulation
  Lyophilized from sterile PBS, pH 7.4
• Background
  Meprin A subunit alpha, also known as MEP1A, and Endopeptidase-2, is a single-pass type I  membrane protein which belongs to the peptidase M12A family. MEP1A contains one EGF-like domain, one MAM domain, and one MATH domain. Meprins are unique plasma membrane and secreted metalloproteinases that are highly regulated at the transcriptional and post-translational levels. Meprin alpha and beta subunits are abundantly expressed in kidney and intestinal epithelial cells, are secreted into the urinary tract and intestinal lumen, and are found in leukocytes and cancer cells under certain conditions. Meprins are capable of proteolytically degrading extracellular matrix proteins, proteolytically processing bioactive proteins, and play a role in inflammatory processes. Meprin A and B are highly regulated, secreted and cell-surface homo- and hetero-oligomeric enzymes. Meprins are abundantly expressed in kidney and intestine. The multidomain alpha and beta subunits have high sequence identity. They have very different substrate specificities, oligomerization potentials and are differentially regulated. Meprin A appears to be an important therapeutic target and urinary excretion appears to be a potential biomarker of acute kidney injury ( AKI ).
• Technical data
• Conjugation
  histidine
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus
• Source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  MEP1A   Protein   His   tag  
• Gene symbol
  MEP1A
• Short name
  Recombinant Mouse MEP1A Protein (His tag)
• Technique
  Recombinant, Mouse, mouses, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
• Host
  mouse
• Label
  His
• Species
  Mouse, Mouses
• Alternative name
  Rec. Mouse MEP1A Protein (histidine detection labelled)
• Alternative technique
  rec, murine

Gene info

• Identity
  HGNC:7015
• Gene
  MEP1A
• Long gene name
  meprin A subunit alpha
• Synonyms gene name
  • meprin A, alpha (PABA peptide hydrolase)
• Synonyms
  • PPHA
• Synonyms name
  • PABA peptide hydrolase
• Locus
  6p12.3
• Discovery year
  1994-07-19
• Entrez gene record
  4224
• Pubmed identfication
  • 7774936
• RefSeq identity
  • NM_005588
• Classification
  • Astacins
• VEGA ID
  OTTHUMG00000014790

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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