Recombinant Mouse Gremlin 1 / GREM1 Protein (His tag) 100µg
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Catalog number
PKSM040914-100µg
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Price
Please ask
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Size
100µg
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Activity
Measured by its ability to inhibit recombinant human BMP4-induced alkaline phosphatase production by MC3T3-E1 cells.The ED50 for this effect is typically 1-7 μg/mL in the presence of 50 ng/mL of recombinant human BMP4.
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Synonym
Cktsf1b1;Drm;Grem;ld
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Sequence
Met1-Asp184
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Fusion tag
C-His
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Accession
O70326
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Expressed Host
Baculovirus-Insect Cells
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Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
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Purity
> 97 % as determined by SDS-PAGE
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Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
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Stability and Storage
Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Mol Mass
19.7 kDa
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AP Mol Mass
25 kDa
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Formulation
Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% gly, 0.5mM EDTA, 3mM DTT
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Background
GREM1 belongs to the DAN family. It contains 1 CTCK (C-terminal cystine knot-like) domain. GREM1 is a cysteine knot-secreted protein and acts as an inhibitor in the TGF beta signaling pathway. It inhibits BMP-2, -4, and -7. Inhibition by grem 1 of BMPs in mice allow the expression of fibroblast growth factors (FGFs) 4 and 8 and Sonic hedgehog (SHH) which are necessary for proper limb development. It interacts with SLIT1 and SLIT2 in a glycosylation-dependent manner. As a cytokine, GREM1 may play an important role during carcinogenesis and metanephric kidney organogenesis, as a BMP antagonist required for early limb outgrowth and patterning in maintaining the FGF4-SHH feedback loop. It down-regulates the BMP4 signaling in a dose-dependent manner. It also acts as inhibitor of monocyte chemotaxis. GREM1 is highly expressed in small intestine, fetal brain and colon.
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Conjugation
histidine
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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Source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
GREM1-AS1, GREM1
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Short name
Recombinant Mouse Gremlin 1 / GREM1 Protein (His tag) 100µg
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Technique
Recombinant, Mouse, mouses, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
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Host
mouse
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Label
His
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Species
Mouse, Mouses
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Alternative name
Rec. Mouse Gremlin 1 / gremlin 1 Protein (histidine detection labelled) 100µg
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Alternative technique
rec, murine
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Alternative to gene target
gremlin 1, GREM1 and IDBG-5193 and ENSG00000166923 and 26585, receptor a this GO nist activity, Extracellular, Grem1 and IDBG-197200 and ENSMUSG00000074934 and 23892, GREM1 and IDBG-648566 and ENSBTAG00000046604 and 539079
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Gene info
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Identity
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Gene
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Long gene name
GREM1 antisense RNA 1
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Locus
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Discovery year
2021-01-06
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Entrez gene record
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RefSeq identity
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Classification
Gene info
MeSH Data
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Name
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Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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