Recombinant Mouse Gremlin 1 / GREM1 Protein (His tag) 100µg

  • Catalog number
    PKSM040914-100µg
  • Price
    Please ask
  • Size
    100µg
  • Activity
    Measured by its ability to inhibit recombinant human BMP4-induced alkaline phosphatase production by MC3T3-E1 cells.The ED50 for this effect is typically 1-7 μg/mL in the presence of 50 ng/mL of recombinant human BMP4.
  • Synonym
    Cktsf1b1;Drm;Grem;ld
  • Sequence
    Met1-Asp184
  • Fusion tag
    C-His
  • Accession
    O70326
  • Expressed Host
    Baculovirus-Insect Cells
  • Shipping
    In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
  • Purity
    > 97 % as determined by SDS-PAGE
  • Endotoxin
    < 1.0 EU per μg of the protein as determined by the LAL method
  • Stability and Storage
    Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Mol Mass
    19.7 kDa
  • AP Mol Mass
    25 kDa
  • Formulation
    Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% gly, 0.5mM EDTA, 3mM DTT
  • Background
    GREM1 belongs to the DAN family. It contains 1 CTCK (C-terminal cystine knot-like) domain. GREM1 is a cysteine knot-secreted protein and acts as an inhibitor in the TGF beta signaling pathway. It inhibits BMP-2, -4, and -7. Inhibition by grem 1 of BMPs in mice allow the expression of fibroblast growth factors (FGFs) 4 and 8 and Sonic hedgehog (SHH) which are necessary for proper limb development. It interacts with SLIT1 and SLIT2 in a glycosylation-dependent manner. As a cytokine, GREM1 may play an important role during carcinogenesis and metanephric kidney organogenesis, as a BMP antagonist required for early limb outgrowth and patterning in maintaining the FGF4-SHH feedback loop. It down-regulates the BMP4 signaling in a dose-dependent manner. It also acts as inhibitor of monocyte chemotaxis. GREM1 is highly expressed in small intestine, fetal brain and colon.
  • Conjugation
    histidine
  • Test
    Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Latin name
    Mus musculus
  • Source
    Recombinants or rec. proteins
  • Group
    recombinants
  • Gene target
    Gremlin   GREM1   Protein   His   tag   100µg  
  • Gene symbol
    GREM1-AS1, GREM1
  • Short name
    Recombinant Mouse Gremlin 1 / GREM1 Protein (His tag) 100µg
  • Technique
    Recombinant, Mouse, mouses, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
  • Host
    mouse
  • Label
    His
  • Species
    Mouse, Mouses
  • Alternative name
    Rec. Mouse Gremlin 1 / gremlin 1 Protein (histidine detection labelled) 100µg
  • Alternative technique
    rec, murine
  • Alternative to gene target
    gremlin 1, GREM1 and IDBG-5193 and ENSG00000166923 and 26585, receptor a this GO nist activity, Extracellular, Grem1 and IDBG-197200 and ENSMUSG00000074934 and 23892, GREM1 and IDBG-648566 and ENSBTAG00000046604 and 539079
Gene info
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: The initial culturing of cells derived directly from fresh TISSUES.
  • Tree numbers
    • E01.370.225.500.223.500
    • E05.200.500.265.500
    • E05.242.223.500
    • E05.481.500.249.500
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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