Recombinant Mouse CSNK2A1 / CK2A1 Protein (His & GST tag)
-
Catalog number
PKSM040295-20µg
-
Price
Please ask
-
Size
20µg
-
-
Synonym
Csnk2a1-rs4
-
Activity
The specific activity was determined to be 34 nmol/min/mg using casein as substrate.
-
Sequence
Met1-Gln391
-
Fusion tag
N-His & GST
-
Accession
Q60737
-
Expressed Host
Baculovirus-Insect Cells
-
Shipping
Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.Shipment made at ambient temperature may seriously affect the activity of the ordered products.
-
Purity
> 85 % as determined by SDS-PAGE
-
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
-
Stability and Storage
Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Mol Mass
72.9 kDa
-
AP Mol Mass
65 kDa
-
Formulation
Supplied as sterile 20mM Tris, 500mM NaCl, pH 8.0, 10% gly
-
Background
Casein kinase II subunit alpha, also known as CK II alpha, CSNK2A1 and CK2A1, is a member of the protein kinase superfamily, Ser / Thr protein kinase family and CK2 subfamily. Casein kinase II (CSNK2A1) is a serine / threonine protein kinase that phosphorylates acidic proteins such as casein. This kinase is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. Casein kinase II (CSNK2A1) is a constitutively active, ubiquitously expressed serine / threonine protein kinase that is thought to have a regulatory function in cell proliferation, cell differentiation and apoptosis. CSNK2A1 functions as a tetrameric complex consisting of two regulatory beta-subunits and two catalytic units (alpha and alpha') in a homomeric or heteromeric conformation. Whilst the alpha- and alpha'-subunits are catalytically identical, proteins that regulate CSNK2A1, such as cdc2 and Hsp90, preferentially bind to the alpha and not the alpha'-subunit. CSNK2A1 can phosphorylate a number of key intracellular signaling proteins implicated in tumor suppression (p53 and PTEN) and tumorigenesis (myc, jun, NF-kappaB). CSNK2A1 is also thought to influence Wnt signaling via beta-catenin phosphorylation and the PI 3-K signaling pathway via th phosphorylation of Akt.
-
-
Conjugation
histidine
-
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
-
Latin name
Mus musculus
-
Source
Recombinants or rec. proteins
-
Group
recombinants
-
Gene target
-
Gene symbol
CSNK2A2, CSNK2A1, MGST3
-
Short name
Recombinant Mouse CSNK2A1 / CK2A1 Protein (His & GST tag)
-
Technique
Recombinant, Mouse, mouses, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
-
Host
mouse
-
Label
His
-
Species
Mouse, Mouses
-
Alternative name
Rec. Mouse CSNK2A1 / CK2A1 Protein (histidine & GST detection labelled)
-
Alternative technique
rec, murine
-
Gene info
-
Identity
-
Gene
-
Long gene name
casein kinase 2 alpha 2
-
Synonyms gene name
- casein kinase 2, alpha prime polypeptide
-
Synonyms
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year
1992-02-13
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene name
casein kinase 2 alpha 1
-
Synonyms gene name
- casein kinase 2, alpha 1 polypeptide
-
Synonyms
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year
1992-02-13
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
VEGA ID
-
Locus Specific Databases
Gene info
MeSH Data
-
Name
-
Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
-
Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
-
Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
Similar products