Recombinant Mouse Carboxypeptidase B1 / CPB1 Protein (His tag) 20µg
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Catalog number
PKSM040708-20µg
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Price
Please ask
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Size
20µg
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Activity
Measured by its ability to cleave a colorimetric peptide substrate, Hippuryl-Arg. The specific activity is >5,000 pmoles/min/μg.
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Synonym
0910001A18Rik;1810063F02Rik;2210008M23Rik;AI504870
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Sequence
Met 1-Tyr 415
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Fusion tag
C-His
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Accession
NP_083982.1
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Expressed Host
HEK293 Cells
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Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
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Purity
> 94 % as determined by SDS-PAGE
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Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
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Stability and Storage
Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Mol Mass
47.5 kDa
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AP Mol Mass
43-45 kDa
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Formulation
Lyophilized from sterile 20mM MES, 150mM NaCl, pH 6.5
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Background
Carboxypeptidase B1, also well known as pancreatic procarboxypeptidase B (PCPB), is a highly pancreas -specific protein (PASP), and has been identified previously as a serum marker for acute pancreatitis and pancreatic graft rejection. As the prototype for those human exopeptidases that cleave off basic C-terminal residues, CPB1 specifically cleaves the C-terminal Arg and Lys residues with a preference for Arg. The B1 and B2 forms of procarboxypeptidase B differ from each other mainly in isoelectric point.The deduced amino acid sequence of PCPB predicts a 416-amino acid preproenzyme consisting of a 15-aa signal peptide, a 95-aa activation peptide and a 307-aa mature chain. The secreted PCPB zymogen is converted to enzymatically active CPB1 by limited proteolysis by trypsin.
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Conjugation
histidine
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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Source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
CPB1
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Short name
Recombinant Mouse Carboxypeptidase B1 / CPB1 Protein (His tag) 20µg
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Technique
Recombinant, Mouse, mouses, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
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Host
mouse
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Label
His
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Species
Mouse, Mouses
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Alternative name
Rec. Mouse Carboxypeptidase B1 / CPB1 Protein (histidine detection labelled) 20µg
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Alternative technique
rec, murine
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Gene info
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Identity
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Gene
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Long gene name
carboxypeptidase B1
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Synonyms gene name
- carboxypeptidase B1 (tissue)
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Synonyms name
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GenBank acession
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Locus
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Discovery year
1992-04-13
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Entrez gene record
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RefSeq identity
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Classification
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VEGA ID
MeSH Data
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Name
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Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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