Recombinant Human Wilms tumor protein(WT1)

Verified reactivity

Homo sapiens (Human)
Protein number

P19544
Gene number

WT1
Other name

WT33
Protein origin

E.coli
Protein region

1-449aa
Protein sequence

MGSDVRDLNALLPAVPSLGGGGGCALPVSGAAQWAPVLDFAPPGASAYGSLGGPAPPPAPPPPPPPPPHSFIKQEPSWGGAEPHEEQCLSAFTVHFSGQFTGTAGACRYGPFGPPPPSQASSGQARMFPNAPYLPSCLESQPAIRNQGYSTVTFDGTPSYGHTPSHHAAQFPNHSFKHEDPMGQQGSLGEQQYSVPPPVYGCHTPTDSCTGSQALLLRTPYSSDNLYQMTSQLECMTWNQMNLGATLKGVAAGSSSSVKWTEGQSNHSTGYESDNHTTPILCGAQYRIHTHGVFRGIQDVRRVPGVAPTLVRSASETSEKRPFMCAYPGCNKRYFKLSHLQMHSRKHTGEKPYQCDFKDCERRFSRSDQLKRHQRRHTGVKPFQCKTCQRKFSRSDHLKTHTRTHTGKTSEKPFSCRWPSCQKKFARSDELVRHHNMHQRNMTKLQLAL
Information about sequence

Full Length
Expected molecular weight

65.2kDa
Protein purity

≥ 90%
Storage recommendation

Aliquot and store at -20°C. Minimize freezing and thawing.
Use before

1 year
Shipping requirements

Blue ice
Estimated production time

7-11 business days
Notes

For research use only. Not for diagnostic procedures.
Hyperlink
Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Source

Recombinants or rec. proteins
Group

recombinants

Gene target

Wilms tumor protein WT1
Gene symbol

WT1-AS, WT1
Short name

Recombinant Wilms tumor protein(WT1)
Technique

Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. bioma advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
Label

N-terminal 6xHis-SUMO-tagged
Species

Human, Humans
Alternative name

Rec. H. sapiens Wilms tumor protein(Wilms tumor 1)
Alternative technique

rec
Alternative to gene target

Wilms tumor 1, AWT1 and EWS-WT1 and GUD and NPHS4 and WAGR and WIT-2 and WT33, WT1 and IDBG-37982 and ENSG00000184937 and 7490, C2H2 zinc finger domain binding, nuclei, Wt1 and IDBG-194872 and ENSMUSG00000016458 and 22431, WT1 and IDBG-636230 and ENSBTAG00000047268 and 782183
Tissue

tumor

Identity

HGNC:18135
Gene

WT1-AS
Long gene name

WT1 antisense RNA
Synonyms gene
- WIT1
Synonyms gene name
- Wilms tumor upstream neighbor 1
- WT1 antisense RNA (non-protein coding)
Synonyms
- WIT-1
- WT1AS
- WT1-AS1
Synonyms name
- Wilms tumor associated protein
GenBank acession
- BC002734
Locus

11p13
Discovery year

2005-10-28
Entrez gene record

51352
Pubmed identfication
- 2173145
- 8406502
- 17210670
RefSeq identity
- NR_023920
Classification
- Antisense RNAs
VEGA ID

OTTHUMG00000039557

Name

Tumor Stem Cell Assay
Concept

Scope note: A cytologic technique for measuring the functional capacity of tumor stem cells by assaying their activity. It is used primarily for the in vitro testing of antineoplastic agents.
Tree numbers
- E01.370.225.500.383.910
- E01.370.225.500.388.930
- E05.200.500.383.910
- E05.200.500.388.930
- E05.242.383.910
- E05.242.417.500
- E05.337.550.200.800
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data