Recombinant Human BMPR2 / BMPR-II Protein (His & Fc tag) 200µg

• Catalog number
  PKSH031533-200µg
• Price
  Please ask
• Size
  200µg

• Synonym
  BMPR-II;BMPR3;BMR2;BRK-3;POVD1;PPH1;T-ALK
• Activity
  Measured by its binding ability in a functional ELISA.2. Immobilized human BMPR-II-Fc at 10 μg/mL (100 μl/well) can bind  biotinylated human BMP2-Fc, The EC50 of biotinylated human BMP2-Fc (Cat:10426-H01H) is 80-110 ng/mL.
• Sequence
  Met 1-Ile 151
• Fusion tag
  C-His & Fc
• Accession
  NP_001195.2
• Expressed Host
  HEK293 Cells
• Shipping
  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
• Purity
  ＞90 % as determined by SDS-PAGE
• Endotoxin
  ＜1.0 EU per µg as determined by the LAL method
• Stability and Storage
  Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Mol Mass
  42 kDa
• AP Mol Mass
  60-65 kDa
• Formulation
  Lyophilized from sterile PBS, pH 7.4
• Reconstitution
  Please refer to it for detailed information.
• Background
  The bone morphogenetic protein type II receptor (BMPR-II, or BMPR2), a receptor for the transforming growth factor (TGF)-beta/bone morphogenetic protein (BMP) superfamily. Reduced expression or function of BMPR2 signaling leads to exaggerated TGF-beta signaling and altered cellular responses to TGF-beta. In endothelial cells, BMPR2 mutation increases the susceptibility of cells to apoptosis. BMPR2 transduces BMP signals by forming heteromeric complexes with and phosphorylating BMP type I receptors. The intracellular domain of BMPR2 is both necessary and sufficient for receptor complex interaction. It had been identified that BMPR2 plays a key role in cell growth. Its mutations lead to hereditary pulmonary hypertension, and knockout of Bmpr-II results in early embryonic lethality. The C-terminal tail of BMPR2 provides binding sites for a number of regulatory proteins that may initiate Smad-independent signalling. BMPR2 mutations were predicted to alter the BMP and TGF-b1/SMAD signalling pathways, resulting in proliferation rather than apoptosis of vascular cells, and greatly increase the risk of developing severe pulmonary arterial hypertension. BMPR2 gene result in familial Primary pulmonary hypertension (PPH) transmitted as an autosomal dominant trait, albeit with low penetrance. Heterozygous germline mutations of BMPR2 gene have been identified in patients with familial and sporadic PPH, indicating that BMPR2 may contribute to the maintenance of normal pulmonary vascular structure and function. Tctex-1, a light chain of the motor complex dynein, interacts with the cytoplasmic domain of BMPR2 and demonstrate that Tctex-1 is phosphorylated by BMPR-II, a function disrupted by PPH disease causing mutations within exon 12. BMPR2 and Tctex-1 co-localize to endothelium and smooth muscle within the media of pulmonary arterioles, key sites of vascular remodelling in PPH.
• Product URL
• PDF URL
• Conjugation
  histidine
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  BMPR2   BMPR-II   Protein   His   tag   200µg  
• Gene symbol
  BMPR2, CRABP2, SPINK2, MGST2, IBSP, APOL2, MGAT2, P4HA2, TMED10P1, XYLT2
• Short name
  Recombinant BMPR2 / BMPR-II Protein (His & Fc tag) 200µg
• Technique
  Recombinant, FC, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
• Label
  His
• Species
  Human, Humans
• Alternative name
  Rec. H. sapiens bone morphogenetic protein receptor, classification II (serine/threonine kinase) / BMPR-II Protein (histidine & fragment c detection labelled) 200µg
• Alternative technique
  rec
• Alternative to gene target
  bone morphogenetic protein receptor, type II (serine/threonine kinase), BMPR-II and BMPR3 and BMR2 and BRK-3 and POVD1 and PPH1 and T-ALK, BMPR2 and IDBG-78880 and ENSG00000204217 and 659, activin receptor activity, Extracellular, Bmpr2 and IDBG-158574 and ENSMUSG00000067336 and 12168, BT.25715 and IDBG-643309 and ENSBTAG00000006420 and 407127

Gene info

• Identity
  HGNC:1078
• Gene
  BMPR2
• Long gene name
  bone morphogenetic protein receptor type 2
• Synonyms gene
  • PPH1
• Synonyms gene name
  • primary pulmonary hypertension 1
  • bone morphogenetic protein receptor, type II (serine/threonine kinase)
  • bone morphogenetic protein receptor type II
• Synonyms
  • BRK-3
  • T-ALK
  • BMPR3
  • BMPR-II
• GenBank acession
  • Z48923
• Locus
  2q33.1-q33.2
• Discovery year
  1997-03-19
• Entrez gene record
  659
• Pubmed identfication
  • 7791754
• RefSeq identity
  • NM_001204
• Classification
  • Type 2 receptor serine/threonine kinases
• VEGA ID
  OTTHUMG00000133617
• Locus Specific Databases
  • LRG_712

Gene info

• Identity
  HGNC:2339
• Gene
  CRABP2
• Long gene name
  cellular retinoic acid binding protein 2
• Synonyms gene name
  • cellular retinoic acid-binding protein 2
• Synonyms
  • CRABP-II
• GenBank acession
  • BC001109
• Locus
  1q23.1
• Discovery year
  1992-11-25
• Entrez gene record
  1382
• Pubmed identfication
  • 1654334
• RefSeq identity
  • NM_001878
• Classification
  • Fatty acid binding protein family
• VEGA ID
  OTTHUMG00000041300

Gene info

• Identity
  HGNC:11245
• Gene
  SPINK2
• Long gene name
  serine peptidase inhibitor Kazal type 2
• Synonyms gene name
  • serine protease inhibitor, Kazal type 2 (acrosin-trypsin inhibitor)
• Synonyms
  • HUSI-II
• Synonyms name
  • acrosin-trypsin inhibitor
• GenBank acession
  • BC022514
• Locus
  4q12
• Discovery year
  1993-12-01
• Entrez gene record
  6691
• Pubmed identfication
  • 8428671
• RefSeq identity
  • NM_021114
• Classification
  • Serine peptidase inhibitors, Kazal type
• VEGA ID
  OTTHUMG00000128769

Gene info

• Identity
  HGNC:7063
• Gene
  MGST2
• Long gene name
  microsomal glutathione S-transferase 2
• Synonyms
  • MGST-II
• GenBank acession
  • U77604
• Locus
  4q31.1
• Discovery year
  1997-10-16
• Entrez gene record
  4258
• Pubmed identfication
  • 8703034
• RefSeq identity
  • NM_002413
• Classification
  • Microsomal glutathione S-transferases
• VEGA ID
  OTTHUMG00000133382

Gene info

• Identity
  HGNC:5341
• Gene
  IBSP
• Long gene name
  integrin binding sialoprotein
• Synonyms
  • BSP
  • SP-II
  • BSP-II
• Synonyms name
  • bone sialoprotein
  • bone sialoprotein II
• Locus
  4q22.1
• Discovery year
  1993-03-23
• Entrez gene record
  3381
• Pubmed identfication
  • 8406493
• Classification
  • SIBLING family
• VEGA ID
  OTTHUMG00000130600

Gene info

• Identity
  HGNC:619
• Gene
  APOL2
• Long gene name
  apolipoprotein L2
• Synonyms
  • APOL-II
• Synonyms name
  • apolipoprotein L-II
• GenBank acession
  • AF324224
• Locus
  22q12.3
• Discovery year
  1999-10-29
• Entrez gene record
  23780
• Pubmed identfication
  • 10591208
  • 11374903
• RefSeq identity
  • NM_145637
• Classification
  • Apolipoproteins
• VEGA ID
  OTTHUMG00000150634

Gene info

• Identity
  HGNC:7045
• Gene
  MGAT2
• Long gene name
  alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase
• Synonyms gene name
  • mannosyl (alpha-1,6-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase
• Synonyms
  • GNT-II
• GenBank acession
  • U15128
• Locus
  14q21.3
• Discovery year
  1993-02-16
• Entrez gene record
  4247
• Pubmed identfication
  • 7635144
• RefSeq identity
  • NM_002408
• Classification
  • Mannosyl-glycoprotein N-acetylglucosaminyltransferases
• VEGA ID
  OTTHUMG00000140271
• Locus Specific Databases
  • Congenital Disorder of Glycosylation pages

Gene info

• Identity
  HGNC:8547
• Gene
  P4HA2
• Long gene name
  prolyl 4-hydroxylase subunit alpha 2
• Synonyms gene name
  • procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide II
  • prolyl 4-hydroxylase, alpha polypeptide II
• Synonyms
  • C-P4Halpha(II)
• Synonyms name
  • 4-PH alpha 2
  • collagen prolyl 4-hydroxylase alpha(II)
• GenBank acession
  • U90441
• Locus
  5q31.1
• Discovery year
  1999-02-23
• Entrez gene record
  8974
• Pubmed identfication
  • 9211872
  • 9149945
• RefSeq identity
  • NM_004199
• Classification
  • MicroRNA protein coding host genes
• VEGA ID
  OTTHUMG00000059647

Gene info

• Identity
  HGNC:30754
• Gene
  TMED10P1
• Long gene name
  transmembrane p24 trafficking protein 10 pseudogene 1
• Synonyms gene
  • TMED10P
• Synonyms gene name
  • transmembrane emp24-like trafficking protein 10 (yeast) pseudogene
  • transmembrane emp24-like trafficking protein 10 (yeast) pseudogene 1
• Synonyms
  • Tmp21-II
• GenBank acession
  • AJ004914
• Locus
  8q24.3
• Discovery year
  2005-08-26
• Entrez gene record
  286102
• Pubmed identfication
  • 10376215
• RefSeq identity
  • XR_002807
• VEGA ID
  OTTHUMG00000165203

Gene info

• Identity
  HGNC:15517
• Gene
  XYLT2
• Long gene name
  xylosyltransferase 2
• Synonyms gene name
  • xylosyltransferase II
• Synonyms
  • XT-II
  • PXYLT2
• Synonyms name
  • protein xylosyltransferase 2
• GenBank acession
  • AJ277442
• Locus
  17q21.33
• Discovery year
  2001-04-06
• Entrez gene record
  64132
• Pubmed identfication
  • 11099377
• RefSeq identity
  • NM_022167
• Classification
  • Glucosaminyl (N-acetyl) transferases/xylosyltransferases
• VEGA ID
  OTTHUMG00000162057

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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