Recombinant Human Siglec-2 / CD22 Protein (aa 176-687, His tag)

• Catalog number
  PKSH030957-50µg
• Price
  Please ask
• Size
  50µg

• Synonym
  SIGLEC-2;SIGLEC2
• Activity
  NA
• Sequence
  Trp176-Arg687
• Fusion tag
  C-His
• Accession
  Q32M46
• Expressed Host
  HEK293 Cells
• Shipping
  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
• Purity
  ＞90 % as determined by SDS-PAGE
• Endotoxin
  ＜1.0 EU per µg as determined by the LAL method
• Stability and Storage
  Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Mol Mass
  58.5 kDa
• AP Mol Mass
  90-100 kDa
• Formulation
  Lyophilized from sterile PBS, pH 7.4
• Background
  CD22 is a member of the immunoglobulin superfamily, SIGLEC family of lectins. It is first expressed in the cytoplasm of pro-B and pre-B cells, and on the surface as B cells mature to become IgD+. CD22 serves as an adhesion receptor for sialic acid-bearing ligands expressed on erythrocytes and all leukocyte classes. In addition to its potential role as a mediator of intercellular interactions, signal transduction through CD22 can activate B cells and modulate antigen receptor signaling in vitro. The phenotype of CD22-deficient mice suggests that CD22 is primarily involved in the generation of mature B cells within the bone marrow, blood, and marginal zones of lymphoid tissues. CD22 recruits the tyrosine phosphatase Src homology 2 domain-containing phosphatase 1 (SHP-1) to immunoreceptor tyrosine-based inhibitory motifs (ITIMs) and inhibits B-cell receptor (BCR)-induced Ca2+ signaling on normal B cells. CD22 interacts specifically with ligands carrying alpha2-6-linked sialic acids. As an inhibitory coreceptor of the B-cell receptor (BCR), CD22 plays a critical role in establishing signalling thresholds for B-cell activation. Like other coreceptors, the ability of CD22 to modulate B-cell signalling is critically dependent upon its proximity to the BCR, and this in turn is governed by the binding of its extracellular domain to alpha2,6-linked sialic acid ligands. However, genetic studies in mice reveal that some CD22 functions are regulated by ligand binding, whereas other functions are ligand-independent and may only require expression of an intact CD22 cytoplasmic domain at the B-cell surface. CD19 regulates CD22 phosphorylation by augmenting Lyn kinase activity, while CD22 inhibits CD19 phosphorylation via SHP-1.Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy
• Technical data
• Conjugation
  histidine
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  Siglec-2   CD22   Protein   176-687   His   tag  
• Gene symbol
  CD22
• Short name
  Recombinant Siglec-2 / CD22 Protein (aa 176-687, His tag)
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
• Label
  His
• Species
  Human, Humans
• Alternative name
  Rec. H. sapiens Siglec-2 / CD22 molecule Protein (aa 176-687, histidine detection labelled)
• Alternative technique
  rec
• Alternative to gene target
  CD22 molecule, SIGLEC-2 and SIGLEC2, CD22 and IDBG-44001 and ENSG00000012124 and 933, protein binding, Cell surfaces, Cd22 and IDBG-171574 and ENSMUSG00000030577 and 12483, CD22 and IDBG-635610 and ENSBTAG00000011887 and 514582

Gene info

• Identity
  HGNC:1643
• Gene
  CD22
• Long gene name
  CD22 molecule
• Synonyms gene name
  • CD22 antigen
• Synonyms
  • SIGLEC-2
  • SIGLEC2
• Synonyms name
  • sialic acid binding Ig-like lectin 2
• GenBank acession
  • X52785
• Locus
  19q13.12
• Discovery year
  1991-06-06
• Entrez gene record
  933
• Pubmed identfication
  • 8496602
  • 1691828
• RefSeq identity
  • NM_001771
• Classification
  • CD molecules
  • MicroRNA protein coding host genes
  • C2-set domain containing
  • Sialic acid binding Ig like lectins
• VEGA ID
  OTTHUMG00000183298

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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