Recombinant Human Semaphorin-5A/SEMA5A (C-6His)

Alternative names

Semaphorin-5A, Semaphorin-F, Sema F, SEMA5A, SEMAF
Description

Recombinant Human Semaphorin 5A is produced by our Mammalian expression system and the target gene encoding Glu23-Thr765 is expressed with a 6His tag at the C-terminus.
Expression

Human Cells
Tag

C-His
Endotoxin

Less than 0.1 ng/ug (1 EU/ug)
Accession

Q13591
TargetSequence

Glu23-Thr765
Shipping

Ambient
Formulation

Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, 0.1mM EDTA, 0.05% Tween 20, pH 7.2.
Note

For research use only.
Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Source

Recombinants or rec. proteins
Group

recombinants

Gene target

Semaphorin-5A/SEMA5A C-6His
Gene symbol

IGHD5OR15-5A, EIF5A, IGKV1OR22-5, TNKS, SEMA5A-AS1, SEMA5A, MIA2
Short name

Recombinant Semaphorin-5A/SEMA5A (C-6His)
Technique

Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Bon Opus advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
Species

Human, Humans
Alternative name

Rec. H. sapiens Semaphorin-5A/sema domain, seven thrombospondin repeats (classification 1 and classification 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (C-6His)
Alternative technique

rec
Alternative to gene target

sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A, SEMAF and semF, SEMA5A and IDBG-12173 and ENSG00000112902 and 9037, syndecan binding, Plasma membranes, Sema5a and IDBG-133049 and ENSMUSG00000022231 and 20356, SEMA5A and IDBG-631754 and ENSBTAG00000005021 and 506636

Identity

HGNC:5773
Gene

IGKV1OR22-5
Long gene name

immunoglobulin kappa variable 1/OR22-5 (pseudogene)
Synonyms gene
- IGKVP7
- IGKV1OR22-5A
Synonyms gene name
- immunoglobulin kappa variable 1/OR22-5
- immunoglobulin kappa variable 1/OR22-5 pseudogene
Synonyms
- IGKV1/OR22-5
- IGKV1/OR22-5A
GenBank acession
- Z00003
Locus

22q11
Discovery year

2000-04-18
Entrez gene record

28850
Pubmed identfication
- 6087279
Classification
- Immunoglobulin kappa (IGK) orphons
VEGA ID

OTTHUMG00000147349

Identity

HGNC:11941
Gene

TNKS
Long gene name

tankyrase
Synonyms gene name
- tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase
Synonyms
- TIN1
- TINF1
- TNKS1
- PARP-5a
- PARP5A
- pART5
- ARTD5
Synonyms name
- TRF1-interacting ankyrin-related ADP-ribose polymerase 1
GenBank acession
- AF082556
Locus

8p23.1
Discovery year

1999-01-08
Entrez gene record

8658
Pubmed identfication
- 9822378
- 10198177
RefSeq identity
- NM_003747
Classification
- Poly(ADP-ribose) polymerases
- Sterile alpha motif domain containing
- Ankyrin repeat domain containing
- MicroRNA protein coding host genes
VEGA ID

OTTHUMG00000090481

Identity

HGNC:10736
Gene

SEMA5A
Long gene name

semaphorin 5A
Synonyms gene
- SEMAF
Synonyms gene name
- sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A
Synonyms
- semF
GenBank acession
- U52840
Locus

5p15.31
Discovery year

1999-06-25
Entrez gene record

9037
Pubmed identfication
- 8817451
- 9464278
Classification
- MicroRNA protein coding host genes
- Semaphorins
VEGA ID

OTTHUMG00000090501

Identity

HGNC:18432
Gene

MIA2
Long gene name

MIA SH3 domain ER export factor 2
Synonyms gene
- CTAGE5
- MGEA
- MGEA6
Synonyms gene name
- CTAGE family member 5, ER export factor
- meningioma expressed antigen 6 (coiled-coil proline-rich)
- CTAGE family member 5
- melanoma inhibitory activity 2
Synonyms
- FLJ22404
- TALI
- MEA6
- cTAGE-5A
- cTAGE-5B
- cTAGE-5C
- cTAGE-5D
- MGEA11
Synonyms name
- TANGO1-like
GenBank acession
- BC035981
Locus

14q13.2
Discovery year

2003-10-02
Entrez gene record

4253
Pubmed identfication
- 12586826
- 27138255
RefSeq identity
- NM_054024
Classification
- MIA family
- CTAGE family
VEGA ID

OTTHUMG00000028831

Name

Primary Cell Culture
Concept

Scope note: The initial culturing of cells derived directly from fresh TISSUES.
Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data