Recombinant Human S100 Calcium Binding Protein A8/A9 Heterodimer (C-6His)
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Catalog numberC796-500
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PricePlease ask
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Size500 ug
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DescriptionRecombinant Human S100A8 & S100A9 Heterodimer is produced by our E.coli expression system and the target gene encoding Met1-Glu93(S100A8)&Thr2-Pro114(S100A9) is expressed with a 6His tag at the C-terminus.
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Species reactivityHuman
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OriginEscherichia coli
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Peptide sequenceMLTELEKALNSIIDVYHKYSLIKGNFHAVYRDDLKKLLETECPQYIRKKGADVWFKELDINTDGAVNFQEFLILVIKMGVAAHKKSHEESHKEHHHHHH&TCKMSQLERNIETIINTFHQYSVKLGHPDTLNQGEFKELVRKDLQNFLKKENKNEKVIEHIMEDLDTNADKQLSFEEFIMLMARLTWASHEKMHEGDEGPGHHHKPGLGEGTP
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Estimated molecular weight11,7&13,2 kDa
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Protein purityGreater than 95% as determined by reducing SDS-PAGE.
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Endotoxin levelLess than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.
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Shipping conditionDry ice/ice packs
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Package formSupplied as a 0.2 µm filtered solution of 20mM HEPES,10%Glycerol,150mM NaCl,2.5mM EDTA,pH 7.4.
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Storage conditionsStore at below -20°C, stable for 6 months after receipt. Please minimize freeze-thaw cycles.
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Reconstitution conditionsAlways centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 µg/ml.Dissolve the lyophilized protein in ddH2O.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
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UniProt numberP05109 & P06702
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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SourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolS100A1, S100Z, S100A8, S100A9
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Short nameRecombinant S100 Calcium Binding Protein A8/A9 Heterodimer (C-6His)
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TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. novo advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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SpeciesHuman, Humans
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Alternative nameHuman S100A8 & S100A9 Heterodimer(C-6His)
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Alternative techniquerec
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Gene info
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Identity
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Gene
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Long gene nameS100 calcium binding protein A1
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Synonyms gene
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Synonyms gene name
- S100 calcium-binding protein A1
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Synonyms
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GenBank acession
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Locus
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Discovery year1991-07-10
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- S100 calcium binding proteins
- EF-hand domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameS100 calcium binding protein Z
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Synonyms gene name
- S100 calcium binding protein, zeta
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Synonyms
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GenBank acession
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Locus
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Discovery year2004-09-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- S100 calcium binding proteins
- EF-hand domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameS100 calcium binding protein A8
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Synonyms gene
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Synonyms gene name
- calgranulin A
- cystic fibrosis antigen
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1989-05-19
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Entrez gene record
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RefSeq identity
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Classification
- S100 calcium binding proteins
- EF-hand domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameS100 calcium binding protein A9
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Synonyms gene
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Synonyms gene name
- calgranulin B
- S100 calcium-binding protein A9 (calgranulin B)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1989-05-19
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Entrez gene record
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RefSeq identity
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Classification
- S100 calcium binding proteins
- EF-hand domain containing
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VEGA ID
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data