NT5C3B, human recombinant

• Catalog number
  P1040-100
• Price
  Please ask
• Size
  100 ug

• Synonyms
  NT5C3L, 7-methylguanosine phosphate-specific 5'-nucleotidase
• Alternative_names
  NT5C3L, 7-methylguanosine phosphate-specific 5'-nucleotidase
• Recombinant
  Yes
• Source
  E. coli
• Purity by SDS PAGE
  > 85%
• Assay
  SDS-PAGE
• Molecular Weight
  36.8 kDa
• Storage Temp
  -20°C
• Shipping
  Gel Pack
• Shelf Life
  12 months
• Appearance
  Liquid
• Physical form description
  Phosphate Buffered Saline (pH7.4) containing 20% glycerol, 1mM DTT
• Background Information
  NT5C3B, also known 7-methylguanosine phosphate-specific 5'-nucleotidase, includes transferase activity and nucleotide binding. Among its related super-pathways are adenosine nucleotides degradation II and Pyrimidine metabolism. Specifically it hydrolyzes 7-methylguanosine monophosphate (m(7)GMP) to 7- methylguanosine and inorganic phosphate. The specific activity for m(7)GMP may protect cells against undesired salvage of m(7)GMP and its incorporation into nucleic acids. Also it has weak activity for CMP. Recombinant human NT5C3B protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography
• Amino acid sequence
  MGSSHHHHHH SSGLVPRGSH MGSMAEEVST LMKATVLMRQ PGRVQEIVGA LRKGGGDRLQ VISDFDMTLS RFAYNGKRCP SSYNILDNSKIISEECRKEL TALLHHYYPI EIDPHRTVKE KLPHMVEWWT KAHNLLCQQK IQKFQIAQVV RESNAMLREG YKTFFNTLYH NNIPLFIFSAGIGDILEEII RQMKVFHPNI HIVSNYMDFN EDGFLQGFKG QLIHTYNKNS SACENSGYFQ QLEGKTNVIL LGDSIGDLTM ADGVPGVQNILKIGFLNDKV EERRERYMDS YDIVLEKDET LDVVNGLLQH ILCQGVQLEM QGP
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  NT5C3B  
• Gene symbol
  NT5C3B
• Short name
  NT5C3B, recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  NT5C3B, H. sapiens Rec.
• Alternative technique
  rec

Gene info

• Identity
  HGNC:28300
• Gene
  NT5C3B
• Long gene name
  5'-nucleotidase, cytosolic IIIB
• Synonyms gene
  • NT5C3L
• Synonyms gene name
  • 5'-nucleotidase, cytosolic III-like
• Synonyms
  • MGC20781
  • cN-IIIB
• Locus
  17q21.2
• Discovery year
  2005-11-15
• Entrez gene record
  115024
• Pubmed identfication
  • 23223233
  • 24603684
• RefSeq identity
  • NM_052935
• VEGA ID
  OTTHUMG00000133499

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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