Recombinant Human Macrophage Migration Inhibitory Factor/MIF
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Catalog number
CH33-500
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Price
Please ask
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Size
500 ug
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Description
Recombinant Human Macrophage migration inhibitory factor is produced by our E.coli expression system and the target gene encoding Met1-Ala115 is expressed.
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Species reactivity
Human
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Origin
Escherichia coli
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Peptide sequence
MPMFIVNTNVPRASVPDGFLSELTQQLAQATGKPPQYIAVHVVPDQLMAFGGSSEPCALCSLHSIGKIGGAQNRSYSKLLCGLLAERLRISPDRVYINYYDMNAANVGWNNSTFA
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Estimated molecular weight
12,5 kDa
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Protein purity
Greater than 95% as determined by reducing SDS-PAGE.
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Endotoxin level
Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.
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Shipping condition
Dry Ice/ice packs
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Package form
Supplied as a 0.2 µm filtered solution of 20mM PB,150mM NaCl,pH7.4.
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Storage conditions
Store at below -20°C, stable for 6 months after receipt. Please minimize freeze-thaw cycles.
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Reconstitution conditions
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 µg/ml.Dissolve the lyophilized protein in ddH2O.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
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UniProt number
P14174
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Additional description
Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
MIF-AS1, MIF, ODC1-DT, DDT, S100A9
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Short name
Recombinant Macrophage Migration Inhibitory Factor/MIF
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. novo advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Species
Human, Humans
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Alternative name
Human MIF
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Alternative technique
rec
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Alternative to gene target
macrophage migration inhibitory factor (glycosylation-inhibiting factor), GIF and GLIF and MMIF, MIF and IDBG-401910 and ENSG00000240972 and 4282, phenylpyruvate tautomerase activity, Extracellular, Mif and IDBG-163956 and ENSMUSG00000033307 and 17319, MIF and IDBG-637094 and ENSBTAG00000007375 and 280858
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Gene info
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Identity
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Gene
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Long gene name
MIF antisense RNA 1
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Synonyms
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Locus
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Discovery year
2014-08-13
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
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VEGA ID
Gene info
Gene info
Gene info
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Identity
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Gene
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Long gene name
D-dopachrome tautomerase
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year
1998-03-06
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
MeSH Data
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Name
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Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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