Recombinant Human IL4R / CD124 Protein (His tag) 100µg

• Catalog number
  PKSH031630-100µg
• Price
  Please ask
• Size
  100µg

• Synonym
  CD124;IL-4RA;IL4R;IL4RA
• Activity
  Measured by its binding ability in a functional ELISA. Immobilized human IL4R-His at 10 μg/mL (100 μl/well) can bind biotinylated human IL4. The EC50 of biotinylated human IL4 is 20.8-48.5 ng/mL.2. Measured by its ability to inhibit IL-4 dependent proliferation of TF-1 human erthroleukemic cells. The ED50 for this effect is 4-20 ng/ml in the presence of 2 ng/ml IL-4.
• Sequence
  Met 1-His 232
• Fusion tag
  C-His
• Accession
  NP_000409.1
• Expressed Host
  HEK293 Cells
• Shipping
  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
• Purity
  ＞98 % as determined by SDS-PAGE
• Endotoxin
  ＜1.0 EU per µg as determined by the LAL method
• Stability and Storage
  Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Mol Mass
  25.3 kDa
• AP Mol Mass
  25.3 kDa
• Formulation
  Lyophilized from sterile PBS, pH 7.4
• Reconstitution
  Please refer to it for detailed information.
• Background
  The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD124, also known as interleukin 4 receptor (IL4R), is a typeⅠ transmembrane protein that can regulate IgE antibody production in B cells through binding to interleukin 4 and interleukin 13 and promote differentiation of Th2 cells through binding to interleukin 4. The membrane-bound form of CD124 can be hydrolyzed to soluble form which can inhibit IL4-mediated cell proliferation and IL5 upregulation by T-cells.
• Product URL
• PDF URL
• Conjugation
  histidine
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  IL4R   CD124   Protein   His   tag   100µg  
• Gene symbol
  IL4R
• Short name
  Recombinant IL4R / CD124 Protein (His tag) 100µg
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
• Label
  His
• Species
  Human, Humans
• Alternative name
  Rec. H. sapiens interleukin 4 receptor / CD124 Protein (histidine detection labelled) 100µg
• Alternative technique
  rec
• Alternative to gene target
  interleukin 4 receptor, CD124 and IL-4RA and IL4RA, IL4R and IDBG-21808 and ENSG00000077238 and 3566, insulin receptor substrate binding, Extracellular, Il4ra and IDBG-209275 and ENSMUSG00000030748 and 16190, IL4R and IDBG-638778 and ENSBTAG00000001602 and 404154

Gene info

• Identity
  HGNC:6015
• Gene
  IL4R
• Long gene name
  interleukin 4 receptor
• Synonyms
  • CD124
• Synonyms name
  • interleukin 13 receptor
• GenBank acession
  • X52425
• Locus
  16p12.1
• Discovery year
  1990-07-06
• Entrez gene record
  3566
• Pubmed identfication
  • 1679753
• Classification
  • Interleukin receptors
  • CD molecules
• VEGA ID
  OTTHUMG00000097015

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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