Recombinant Human IL3RA / CD123 Protein (His & Fc tag)

  • Catalog number
    PKSH031552-20µg
  • Price
    Please ask
  • Size
    20µg
  • Synonym
    CD123;hIL-3Ra;IL3R;IL3RAY;IL3RX;IL3RY
  • Activity
    Measured by its binding ability in a functional ELISA. Immobilized human IL3 at 20 μg/ml (100 μl/well) can bind human IL3RA with a linear ranger of 0.064-1.6 μg/ml.
  • Sequence
    Met 1-Arg 305
  • Fusion tag
    C-His & Fc
  • Accession
    NP_002174.1
  • Expressed Host
    HEK293 Cells
  • Shipping
    In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
  • Purity
    >75 % as determined by SDS-PAGE
  • Endotoxin
    <1.0 EU per µg as determined by the LAL method
  • Stability and Storage
    Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Mol Mass
    61 kDa
  • AP Mol Mass
    80-90 kDa
  • Formulation
    Lyophilized from sterile PBS, pH 7.4
  • Background
    Interleukin-3 receptor subunit alpha, also known as IL-3 receptor subunit alpha, IL-3R-alpha, CD123, and IL3RA, is a single-pass type I membrane protein which belongs to the type I cytokine receptor family and Type 5 subfamily. The specific alpha subunit of the interleukin-3 receptor (IL-3Ralpha, CD123) is strongly expressed in various leukemic blasts and leukemic stem cells and seems to be an excellent target for the therapy of leukemias. The WSXWS motif of IL3RA appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding. The box one motif of IL3RA is required for JAK interaction and / or activation. IL3RA represents a unique marker for primitive leukemic stem cells. Targeting of IL3RA may be a promising strategy for the preferential ablation of AML cells. Aberrant IL3RA expression is a good marker for monitoring of minimal residual disease. IL3RA is strongly expressed in various leukemic blasts and leukemic stem cells and seems to be an excellent target for the therapy of leukemias. Recent studies have shown that interleukin-3 receptor alpha (CD123) is highly expressed on leukemia stem cells of patients with acute myeloid leukemia, and is correlated with tumor load and poor prognosis. CD123 was highly expressed in the bone marrow of the patients with myelodysplastic syndrome (MDS), significantly correlated with the proportion of bone marrow blasts, and thus might be the marker of MDS malignant clone. IL3RA is also a useful new marker for distinguishing B-cell disorders with circulating villous lymphocytes as its expression is characteristic of typical hairy cell leukemia (HCL) with high sensitivity and specificity.
  • Conjugation
    histidine
  • Properties
    Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • Source
    Recombinants or rec. proteins
  • Group
    recombinants
  • Gene target
    IL3RA   CD123   Protein   His   tag  
  • Gene symbol
    IL3RA
  • Short name
    Recombinant IL3RA / CD123 Protein (His & Fc tag)
  • Technique
    Recombinant, FC, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
  • Label
    His
  • Species
    Human, Humans
  • Alternative name
    Rec. H. sapiens interleukin 3 receptor, alpha (low affinity) / CD123 Protein (histidine & fragment c detection labelled)
  • Alternative technique
    rec
  • Alternative to gene target
    interleukin 3 receptor, alpha (low affinity), CD123 and hIL-3Ra and IL3R and IL3RAY and IL3RX and IL3RY, IL3RA and IDBG-39718 and ENSG00000185291 and 3563, protein binding, Plasma membranes, Il3ra and IDBG-132811 and ENSMUSG00000068758 and 16188
Gene info
  • Identity
  • Gene
  • Long gene name
    interleukin 3 receptor subunit alpha
  • Synonyms gene name
    • interleukin 3 receptor, alpha (low affinity)
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    1992-10-28
  • Entrez gene record
  • Pubmed identfication
  • Classification
    • Pseudoautosomal region 1
    • CD molecules
    • Interleukin receptors
  • VEGA ID
MeSH Data
  • Name
  • Concept
    Scope note: The initial culturing of cells derived directly from fresh TISSUES.
  • Tree numbers
    • E01.370.225.500.223.500
    • E05.200.500.265.500
    • E05.242.223.500
    • E05.481.500.249.500
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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