Recombinant Human Hepatocyte Growth Factor/HGF/Hepatopoietin-A (C-6His)
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Catalog numberCJ72-1000
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PricePlease ask
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Size1 mg
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DescriptionRecombinant Human Hepatocyte Growth Factor is produced by our Mammalian expression system and the target gene encoding Gln32-Ser728 is expressed with a 6His tag at the C-terminus.
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Species reactivityHuman
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OriginHuman Cells
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Peptide sequenceQRKRRNTIHEFKKSAKTTLIKIDPALKIKTKKVNTADQCANRCTRNKGLPFTCKAFVFDKARKQCLWFPFNSMSSGVKKEFGHEFDLYENKDYIRNCIIGKGRSYKGTVSITKSGIKCQPWSSMIPHEHSFLPSSYRGKDLQENYCRNPRGEEGGPWCFTSNPEVRYEVCDIPQCSEVECMTCNGESYRGLMDHTESGKICQRWDHQTPHRHKFLPERYPDKGFDDNYCRNPDGQPRPWCYTLDPHTRWEYCAIKTCADNTMNDTDVPLETTECIQGQGEGYRGTVNTIWNGIPCQRWDSQYPHEHDMTPENFKCKDLRENYCRNPDGSESPWCFTTDPNIRVGYCSQIPNCDMSHGQDCYRGNGKNYMGNLSQTRSGLTCSMWDKNMEDLHRHIFWEPDASKLNENYCRNPDDDAHGPWCYTGNPLIPWDYCPISRCEGDTTPTIVNLDHPVISCAKTKQLRVVNGIPTRTNIGWMVSLRYRNKHICGGSLIKESWVLTARQCFPSRDLKDYEAWLGIHDVHGRGDEKCKQVLNVSQLVYGPEGSDLVLMKLARPAVLDDFVSTIDLPNYGCTIPEKTSCSVYGWGYTGLINYDGLLRVAHLYIMGNEKCSQHHRGKVTLNESEICAGAEKIGSGPCEGDYGGPLVCEQHKMRMVLGVIVPGRGCAIPNRPGIFVRVAYYAKWIHKIILTYKVPQSVDHHHHHH
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Estimated molecular weight80,7 kDa
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Protein purityGreater than 95% as determined by reducing SDS-PAGE.
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Endotoxin levelLess than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.
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Shipping conditionAmbient/Room Temperature
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Package formLyophilized from a 0.2 µm filtered solution of 20mM Tris,150mM NaCl,pH8.0.
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Storage conditionsLyophilized protein should be stored at below -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at below -20°C for 3 months.
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Reconstitution conditionsAlways centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 µg/ml.Dissolve the lyophilized protein in ddH2O.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
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UniProt numberP14210
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Additional descriptionAplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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SourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolSOS1, HGF, IL6
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Short nameRecombinant Hepatocyte Growth Factor/HGF/Hepatopoietin-A (C-6His)
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TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. novo advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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SpeciesHuman, Humans
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Alternative nameHuman Hepatocyte growth factor/HGF(C-6His)
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Alternative techniquerec
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Alternative to gene targethepatocyte growth factor (hepapoietin A; scatter factor), DFNB39 and F-TCF and HGFB and HPTA and SF, HGF and IDBG-24150 and ENSG00000019991 and 3082, protein heterodimerization activity, Extracellular, Hgf and IDBG-136449 and ENSMUSG00000028864 and 15234, HGF and IDBG-631194 and ENSBTAG00000017664 and 282879
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Gene info
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Identity
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Gene
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Long gene nameSOS Ras/Rac guanine nucleotide exchange factor 1
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Synonyms gene
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Synonyms gene name
- gingival fibromatosis, hereditary, 1
- son of sevenless homolog 1 (Drosophila)
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Synonyms
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GenBank acession
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Locus
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Discovery year1993-10-27
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Dbl family Rho GEFs
- Pleckstrin homology domain containing
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene namehepatocyte growth factor
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Synonyms gene
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Synonyms gene name
- deafness, autosomal recessive 39
- hepatocyte growth factor (hepapoietin A; scatter factor)
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Synonyms
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Synonyms name
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Locus
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Discovery year1991-06-07
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameinterleukin 6
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Synonyms gene
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Synonyms gene name
- interleukin 6 (interferon, beta 2)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1986-01-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Interleukins
- Interferons
- Interleukin 6 type cytokine family
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VEGA ID
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data