Recombinant Human GADD45a/GADD45A (N-6His)
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Catalog number
CE65-1000
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Price
Please ask
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Size
1 mg
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Description
Recombinant Human GADD45A is produced by our E.coli expression system and the target gene encoding Met1-Arg165 is expressed with a 6His tag at the N-terminus.
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Species reactivity
Human
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Origin
Escherichia coli
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Peptide sequence
MGSSHHHHHHSSGLVPRGSHMTLEEFSAGEQKTERMDKVGDALEEVLSKALSQRTITVGVYEAAKLLNVDPDNVVLCLLAADEDDDRDVALQIHFTLIQAFCCENDINILRVSNPGRLAELLLLETDAGPAASEGAEQPPDLHCVLVTNPHSSQWKDPALSQLICFCRESRYMDQWVPVINLPER
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Estimated molecular weight
20,5 kDa
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Protein purity
Greater than 95% as determined by reducing SDS-PAGE.
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Endotoxin level
Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.
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Shipping condition
Ambient/Room Temperature
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Package form
Lyophilized from a 0.2 µm filtered solution of 20mM PB, 150mM NaCl, pH 7.2.
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Storage conditions
Lyophilized protein should be stored at below -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at below -20°C for 3 months.
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Reconstitution conditions
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 µg/ml.Dissolve the lyophilized protein in ddH2O.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
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UniProt number
P24522
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
GADD45A
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Short name
Recombinant GADD45a/GADD45A (N-6His)
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. novo advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Species
Human, Humans
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Alternative name
Human GADD45A(N-6His)
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Alternative technique
rec
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Alternative to gene target
growth arrest and DNA-damage-inducible, alpha, DDIT1 and GADD45, GADD45A and IDBG-99580 and ENSG00000116717 and 1647, protein binding, nuclei, Gadd45a and IDBG-153723 and ENSMUSG00000036390 and 13197, GADD45A and IDBG-638096 and ENSBTAG00000013860 and 505463
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Gene info
MeSH Data
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Name
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Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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