Recombinant Human Fibroblast Growth Factor 21/FGF-21 (N-6His)

• Catalog number
  C223-500
• Price
  Please ask
• Size
  500 ug

• Description
  Recombinant Human Fibroblast Growth Factor 21 is produced by our E.coli expression system and the target gene encoding His29-Ser209 is expressed with a 6His tag at the N-terminus.
• Species reactivity
  Human
• Origin
  Escherichia coli
• Peptide sequence
  MGSSHHHHHHSSGLVPRGSHMHPIPDSSPLLQFGGQVRQRYLYTDDAQQTEAHLEIREDGTVGGAADQSPESLLQLKALKPGVIQILGVKTSRFLCQRPDGALYGSLHFDPEACSFRELLLEDGYNVYQSEAHGLPLHLPGNKSPHRDPAPRGPARFLPLPGLPPAPPEPPGILAPQPPDVGSSDPLSMVGPSQGRSPSYAS
• Estimated molecular weight
  21,69 kDa
• Protein purity
  Greater than 95% as determined by reducing SDS-PAGE.
• Endotoxin level
  Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.
• Shipping condition
  Ambient/Room Temperature
• Package form
  Lyophilized from a 0.2 µm filtered solution of 20mM Tris, 100mM NaCl, 2mM EDTA, pH9.0 .
• Storage conditions
  Lyophilized protein should be stored at below -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at below -20°C for 3 months.
• Reconstitution conditions
  Always centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 µg/ml.Dissolve the lyophilized protein in ddH2O.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• UniProt number
  Q9NSA1
• Other related products
• Additional description
  Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  Fibroblast   Growth   Factor   21/FGF-21   N-6His  
• Gene symbol
  FGF21, PIRC105, SNORD115-21, HTOR, FGF18, SNORD116-21, SNORD114-21, FGF17
• Short name
  Recombinant Fibroblast Growth Factor 21/FGF-21 (N-6His)
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. novo advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  Human FGF-21(N-6His)
• Alternative technique
  rec

Gene info

• Identity
  HGNC:3678
• Gene
  FGF21
• Long gene name
  fibroblast growth factor 21
• GenBank acession
  • AB021975
• Locus
  19q13.33
• Discovery year
  1999-11-26
• Entrez gene record
  26291
• Pubmed identfication
  • 10858549
• Classification
  • Fibroblast growth factor family
• VEGA ID
  OTTHUMG00000183326

Gene info

• Identity
  HGNC:37609
• Gene
  PIRC105
• Long gene name
  piwi-interacting RNA cluster 105
• Locus
  21
• Discovery year
  2009-11-05
• Entrez gene record
  100313936
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

Gene info

• Identity
  HGNC:33040
• Gene
  SNORD115-21
• Long gene name
  small nucleolar RNA, C/D box 115-21
• Synonyms
  • HBII-52-21
• GenBank acession
  • AF250841
• Locus
  15q11.2
• Discovery year
  2007-02-15
• Entrez gene record
  100033603
• Pubmed identfication
  • 11106375
  • 11726556
• RefSeq identity
  • NR_003313.1
• Classification
  • Small nucleolar RNAs, C/D box

Gene info

• Identity
  HGNC:5285
• Gene
  HTOR
• Long gene name
  5-hydroxytryptamine (serotonin) oxygenase regulator
• Locus
  21
• Discovery year
  2001-06-22
• Entrez gene record
  3349
• Pubmed identfication
  • 93675

Gene info

• Identity
  HGNC:3674
• Gene
  FGF18
• Long gene name
  fibroblast growth factor 18
• Synonyms
  • FGF-18
  • ZFGF5
• GenBank acession
  • AB007422
• Locus
  5q35.1
• Discovery year
  1998-12-22
• Entrez gene record
  8817
• Pubmed identfication
  • 9660775
  • 9742123
• RefSeq identity
  • NM_033649
  • NM_003862
• Classification
  • Fibroblast growth factor family
• VEGA ID
  OTTHUMG00000130464

Gene info

• Identity
  HGNC:33087
• Gene
  SNORD116-21
• Long gene name
  small nucleolar RNA, C/D box 116-21
• Synonyms
  • HBII-85-21
• GenBank acession
  • AF250841
• Locus
  15q11.2
• Discovery year
  2007-02-15
• Entrez gene record
  100033432
• Pubmed identfication
  • 11106375
  • 11726556
• RefSeq identity
  • NR_003335
• Classification
  • Small nucleolar RNAs, C/D box

Gene info

• Identity
  HGNC:33009
• Gene
  SNORD114-21
• Long gene name
  small nucleolar RNA, C/D box 114-21
• Synonyms
  • 14q(II-21)
• Locus
  14q32.31
• Discovery year
  2006-09-26
• Entrez gene record
  767599
• Pubmed identfication
  • 12045206
• RefSeq identity
  • NR_003214.1
• Classification
  • Small nucleolar RNAs, C/D box

Gene info

• Identity
  HGNC:3673
• Gene
  FGF17
• Long gene name
  fibroblast growth factor 17
• Synonyms
  • FGF-13
• GenBank acession
  • AB009249
• Locus
  8p21.3
• Discovery year
  1998-12-22
• Entrez gene record
  8822
• Pubmed identfication
  • 9514906
  • 9751161
• RefSeq identity
  • NM_003867
• Classification
  • Fibroblast growth factor family
• VEGA ID
  OTTHUMG00000097051

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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