Recombinant Human DDR2 Kinase / CD167b Protein (aa 422-855, His & GST tag) 50µg
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Catalog number
PKSH030420-50µg
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Price
Please ask
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Size
50µg
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Synonym
CD167;MIG20a;NTRKR3;TKT;TYRO10
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Activity
The specific activity was determined to be 8 nmol/min/mg using synthetic AXLtide peptide(CKKSRGDYMTMQIG) as substrate.
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Sequence
Arg422-Glu855
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Fusion tag
N-His & GST
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Accession
Q16832
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Expressed Host
Baculovirus-Insect Cells
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Shipping
Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.Shipment made at ambient temperature may seriously affect the activity of the ordered products.
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Purity
>95 % as determined by SDS-PAGE
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Endotoxin
<1.0 EU per µg as determined by the LAL method
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Stability and Storage
Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Mol Mass
77.1 kDa
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AP Mol Mass
77 kDa
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Formulation
Supplied as sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% glycerol
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Reconstitution
Please refer to it for detailed information.
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Background
Discoidin domain receptor 2 (DDR2) or CD167b (cluster of differentiation 167b) is a kind of protein tyrosine kinases associated with cell proliferation and tumor metastasis, and collagen, identified as a ligand for DDR2, up-regulates matrix metallloproteinase 1 (MMP-1) and MMP-2 expression in cellular matrix. DDR2/CD167b was found to recognise the triple-helical region of collagen X as well as the NC1 domain. Binding to the collagenous region was dependent on the triple-helical conformation. DDR2/CD167b autophosphorylation was induced by the collagen X triple-helical region but not the NC1 domain, indicating that the triple-helical region of collagen X contains a specific DDR2 binding site that is capable of receptor activation. DDR2/CD167b is induced during stellate cell activation and implicate the phosphorylated receptor as a mediator of MMP-2 release and growth stimulation in response to type I collagen. Moreover, type I collagen-dependent upregulation of DDR2/CD167b expression establishes a positive feedback loop in activated stellate cells, leading to further proliferation and enhanced invasive activity.Immune Checkpoint Immunotherapy Cancer Immunotherapy Targeted Therapy
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Conjugation
histidine
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
MGST3, DDR2
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Short name
Recombinant DDR2 Kinase / CD167b Protein (aa 422-855, His & GST tag) 50µg
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
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Label
His
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Species
Human, Humans
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Alternative name
Rec. H. sapiens discoidin domain receptor tyrosine kinase 2 phosphorylation catalyst / CD167b Protein (aa 422-855, histidine & GST detection labelled) 50µg
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Alternative technique
rec
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Alternative to gene target
discoidin domain receptor tyrosine kinase 2, MIG20a and NTRKR3 and TKT and TYRO10, DDR2 and IDBG-104396 and ENSG00000162733 and 4921, transferase activity, Plasma membranes, Ddr2 and IDBG-203608 and ENSMUSG00000026674 and 18214, BT.46641 and IDBG-629865 and ENSBTAG00000004885 and 533523
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Gene info
Gene info
MeSH Data
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Name
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Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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