Recombinant Human Malate Dehydrogenase, Cytoplasmic/MDH1 (C-6His)
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Catalog number
C276-50
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Price
Please ask
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Size
50 ug
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Description
Recombinant Human Malate Dehydrogenase is produced by our E.coli expression system and the target gene encoding Ser2-Ala334 is expressed with a 6His tag at the C-terminus.
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Species reactivity
Human
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Origin
Escherichia coli
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Peptide sequence
SEPIRVLVTGAAGQIAYSLLYSIGNGSVFGKDQPIILVLLDITPMMGVLDGVLMELQDCALPLLKDVIATDKEDVAFKDLDVAILVGSMPRREGMERKDLLKANVKIFKSQGAALDKYAKKSVKVIVVGNPANTNCLTASKSAPSIPKENFSCLTRLDHNRAKAQIALKLGVTANDVKNVIIWGNHSSTQYPDVNHAKVKLQGKEVGVYEALKDDSWLKGEFVTTVQQRGAAVIKARKLSSAMSAAKAICDHVRDIWFGTPEGEFVSMGVISDGNSYGVPDDLLYSFPVVIKNKTWKFVEGLPINDFSREKMDLTAKELTEEKESAFEFLSSALEHHHHHH
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Estimated molecular weight
37,5 kDa
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Protein purity
Greater than 95% as determined by reducing SDS-PAGE.
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Endotoxin level
Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.
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Shipping condition
Dry ice/ice packs
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Package form
Supplied as a 0.2 µm filtered solution of 20mM Tris, 150mM NaCl, pH 8.0.
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Storage conditions
Store at below -20°C, stable for 6 months after receipt. Please minimize freeze-thaw cycles.
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Reconstitution conditions
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 µg/ml.Dissolve the lyophilized protein in ddH2O.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
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UniProt number
P40925
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
MDH1
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Short name
Recombinant Malate Dehydrogenase, Cytoplasmic/MDH1 (C-6His)
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. novo advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Species
Human, Humans
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Alternative name
Human Malate Dehydrogenase/MDH1(C-6His)
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Alternative technique
rec
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Gene info
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Identity
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Gene
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Long gene name
malate dehydrogenase 1
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Synonyms gene name
- malate dehydrogenase 1, NAD (soluble)
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Locus
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Discovery year
2001-06-22
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Entrez gene record
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RefSeq identity
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VEGA ID
MeSH Data
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Name
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Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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