Recombinant Human CD32a / FCGR2A Protein (167 Arg, His tag) 100µg

  • Catalog number
    PKSH030295-100µg
  • Price
    Please ask
  • Size
    100µg
  • Synonym
    CD32;CD32A;CDw32;Fc gamma RIIA;FCG2;FcGR;FCGR2;FCGR2A1;IGFR2
  • Activity
    Measured by its binding ability in a functional ELISA. Immobilized recombinant human CD32a at 10 μg/ml (100 μl/well) can bind human IgG2 with a linear range of 12.5-200 ng/ml.
  • Sequence
    Met 1-Ile 218
  • Fusion tag
    C-His
  • Accession
    AAA35827.1
  • Expressed Host
    HEK293 Cells
  • Shipping
    In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
  • Purity
    >97 % as determined by SDS-PAGE
  • Endotoxin
    <1.0 EU per µg as determined by the LAL method
  • Stability and Storage
    Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Mol Mass
    22 kDa
  • AP Mol Mass
    30 kDa
  • Formulation
    Lyophilized from sterile PBS, pH 7.5
  • Reconstitution
    Please refer to it for detailed information.
  • Background
    Receptors for the Fc region of IgG (FcγR) are members of the Ig superfamily that function in the activation or inhibition of immune responses. Human FcγRs are divided into three classes designated FcγRI (CD64), FcγRII (CD32), and FcγRIII (CD16), which generate multiple isoforms, are recognized. The activating- type receptor either has or associates non-covalently with an accessory subunit that has an immunoreceptor tyrosine-based activation motif (ITAM) in its cytoplasmic domain. FcγRI binds IgG with high affinity and functions during early immune responses, whereas FcγRII and RIII are low affinity receptors that recognize IgG as aggregates surrounding multivalent antigens during late immune responses. Three genes for human FcγRII (A, B, and C) and one for mouse (FcγRIIB), encoding type I transmembrane proteins with ITAM motifs (FcγRII A and C) or ITIM motifs (FcγRIIB) in their cytoplasmic domains, have been identified. Human CD32, also known as Low affinity immunoglobulin γ Fc region receptor II-a (IgG Fc receptor II-a), FcγRII A or FCGR2A Protein, is expressed on cells of both myeloid and lymphoid lineages as well as on cells of non-hematopoietic origin. Associated with an ITAM-bearing adapter subunit, FcRγ, CD32a (FcγRII A) delivers an activating signal upon ligand binding, and results in the initiation of inflammatory responses including cytolysis, phagocytosis, degranulation, and cytokine production. The responses can be modulated by signals from the co-expressed inhibitory receptors such as Fcγ RII B, and the strength of the signal is dependent on the ratio of expression of the activating and inhibitory receptors.
  • Conjugation
    histidine
  • Properties
    Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • Source
    Recombinants or rec. proteins
  • Group
    recombinants
  • Gene target
    CD32a   FCGR2A   Protein   167   Arg   His   tag   100µg  
  • Gene symbol
    FCGR2A
  • Short name
    Recombinant CD32a / FCGR2A Protein (167 Arg, His tag) 100µg
  • Technique
    Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
  • Label
    His
  • Species
    Human, Humans
  • Alternative name
    Rec. H. sapiens CD32a / fragment c fragment on Immunoglobulin G, low affinity IIa, receptor (CD32) Protein (167 L-arginine, histidine detection labelled) 100µg
  • Alternative technique
    rec
  • Alternative to gene target
    Fc fragment of IgG, low affinity IIa, receptor (CD32), CD32 and CD32A and CDw32 and FCG2 and FcGR and FCGR2 and FCGR2A1 and IGFR2, FCGR2A and IDBG-104282 and ENSG00000143226 and 2212,9103, IgG binding, Plasma membranes
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: The initial culturing of cells derived directly from fresh TISSUES.
  • Tree numbers
    • E01.370.225.500.223.500
    • E05.200.500.265.500
    • E05.242.223.500
    • E05.481.500.249.500
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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