Recombinant Human CD111 / Nectin-1 / PVRL1 Protein (Fc tag) 100µg
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Catalog number
PKSH031066-100µg
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Price
Please ask
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Size
100µg
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Synonym
CD111;CLPED1;ED4;HIgR;HV1S;HVEC;nectin-1;OFC7;PRR;PRR1;PVRR;PVRR1;SK-12
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Activity
Measured by its binding ability in a functional ELISA. Immobilized rh PVRL3 at 1 μg/ml (100 μl/well) can bind recombinant human Nectin-1 with a linear range of 0.156-5 ng/ml.
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Sequence
Met 1-Thr 334
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Fusion tag
C-Fc
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Accession
NP_002846.3
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Expressed Host
HEK293 Cells
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Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
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Purity
＞98 % as determined by SDS-PAGE
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Endotoxin
＜1.0 EU per µg as determined by the LAL method
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Stability and Storage
Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Mol Mass
61 kDa
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AP Mol Mass
75-85 kDa
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Formulation
Lyophilized from sterile PBS, pH 7.4
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Reconstitution
Please refer to it for detailed information.
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Background
Poliovirus receptor-related 1 (herpesvirus entry mediator C; nectin-1; CD111), also known as PVRL1 is a cell adhesion molecule belonging to the immunoglobulin superfamily that can bind to virion glycoprotein D (gD) to mediate entry of herpes simplex viruses (HSV) and pseudorabies virus (PRV). CD111/Nectin-1/PVRL1 colocalizes with E-cadherin at adherens junctions in epithelial cells. The disruption of cell junctions can result in the redistribution of nectin-1. To determine whether disruption of junctions by calcium depletion influenced the susceptibility of epithelial cells to viral entry, Madin-Darby canine kidney cells expressing endogenous nectin-1 or transfected human nectin-1 were tested for the ability to bind soluble forms of viral gD and to be infected by HSV and PRV, before and after calcium depletion. It has been revealed that binding of HSV and PRV gD was localized to adherens junctions in cells maintained in normal medium but was distributed, along with nectin-1, over the entire cell surface after calcium depletion. Both the binding of gD and the fraction of cells that could be infected by HSV-1 and PRV were enhanced by calcium depletion. Taken together, CD111/Nectin-1/PVRL1 confined to adherens junctions in epithelial cells is not very accessible to virus, whereas dissociation of cell junctions releases nectin-1 to serve more efficiently as an entry recptor.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
NECTIN1
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Short name
Recombinant CD111 / Nectin-1 / PVRL1 Protein (Fc tag) 100µg
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Technique
Recombinant, FC, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
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Label
Fc Tag
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Species
Human, Humans
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Alternative name
Rec. H. sapiens CD111 / Nectin-1 / poliovirus receptor-related 1 (herpesvirus entry mediator C) Protein (fragment c detection labelled) 100µg
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Alternative technique
rec
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Alternative to gene target
poliovirus receptor-related 1 (herpesvirus entry mediator C), CD111 and CLPED1 and ED4 and HIgR and HV1S and HVEC and nectin-1 and OFC7 and PRR and PRR1 and PVRR and PVRR1 and SK-12, PVRL1 and IDBG-74533 and ENSG00000110400 and 5818, cell adhesion molecule binding, Extracellular, Pvrl1 and IDBG-155297 and ENSMUSG00000032012 and 58235, PVRL1 and IDBG-631966 and ENSBTAG00000015939 and 533492
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Gene info
MeSH Data
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Name
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Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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