Recombinant Human BLMH / BLM hydrolase Protein (His tag)
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Catalog number
PKSH031145-10µg
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Price
Please ask
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Size
10µg
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Synonym
BH;BMH
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Activity
Measured by its ability to hydrolyze Met-AMC. The specific activity is >500 pmoles/min/μg.
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Sequence
Ser 2-Glu 455
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Fusion tag
N-His
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Accession
NP_000377.1
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Expressed Host
E. coli
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Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
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Purity
>88 % as determined by SDS-PAGE
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Endotoxin
Please contact us for more information.
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Stability and Storage
Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Mol Mass
53.4 kDa
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AP Mol Mass
53.4 kDa
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Formulation
Lyophilized from sterile PBS, pH 7.4
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Background
The papain superfamily member bleomycin hydrolase (BLMH) is a cytoplasmic cysteine peptidase that is highly conserved through evolution. The only known activity of the enzyme is metabolic inactivation of the glycopeptide bleomycin (BLM), an essential component of combination chemotherapy regimens for cancer. The papain superfamily member bleomycin hydrolase (BLMH) is a neutral cysteine protease with structural similarity to a 20S proteasome. Bleomycin (BLM), a clinically used glycopeptide anticancer agent. BLMH is an essential protectant against BLM-induced death and has an important role in neonatal survival and in maintaining epidermal integrity. Sequencing revealed several putative sites phosphorylated by different types of protein kinases, but no signal sequence, transmembrane domain, N-linked glycosylation site or DNA-binding motif.
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Conjugation
histidine
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
BLMH, BLM
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Short name
Recombinant BLMH / BLM hydrolase Protein (His tag)
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
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Label
His
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Species
Human, Humans
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Alternative name
Rec. H. sapiens BLMH / Bloom syndrome, RecQ helicase-like hydrolase Protein (histidine detection labelled)
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Alternative technique
rec
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Alternative to gene target
Bloom syndrome, RecQ helicase-like, BS and RECQ2 and RECQL2 and RECQL3, BLM and IDBG-30365 and ENSG00000197299 and 641, hydrolase activity, nuclei, Blm and IDBG-195418 and ENSMUSG00000030528 and 12144, BLM and IDBG-629490 and ENSBTAG00000020301 and 534148
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Gene info
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Identity
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Gene
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Long gene name
bleomycin hydrolase
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Synonyms
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GenBank acession
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Locus
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Discovery year
1998-03-11
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
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VEGA ID
Gene info
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Identity
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Gene
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Long gene name
BLM RecQ like helicase
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Synonyms gene name
- Bloom syndrome
- Bloom syndrome RecQ like helicase
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Synonyms
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GenBank acession
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Locus
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Discovery year
1992-11-06
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Entrez gene record
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Pubmed identfication
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Classification
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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