Recombinant Human APEX1 / AP / APEx / Ref-1 Protein (His tag)

• Catalog number
  PKSH030851-20µg
• Price
  Please ask
• Size
  20µg

• Synonym
  APE;APE1;APEN;APEX;APX;HAP1;REF1
• Activity
  NA
• Sequence
  Pro2-Leu 318
• Fusion tag
  N-His
• Accession
  P27695
• Expressed Host
  E. coli
• Shipping
  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
• Purity
  ＞92 % as determined by SDS-PAGE
• Endotoxin
  ＜1.0 EU per µg as determined by the LAL method
• Stability and Storage
  Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Mol Mass
  37 kDa
• AP Mol Mass
  37 kDa
• Formulation
  Lyophilized from sterile PBS, pH 7.5
• Background
  The enzyme is known to be a redox factor (Ref-1) stimulating DNA binding activity of AP-1 binding proteins such as Fos and Jun as well as a multifunctional DNA repair enzyme having 5' AP endonuclease, DNA 3' repair diesterase, 3'-5' exonuclease and DNA 3'-phosphatase activities.Although Apex mRNA was expressed ubiquitously, the levels varied significantly, suggesting organ- or tissue-specific expression of the Apex gene. The highest level was observed in the testis, relatively high levels in the thymus, spleen, kidney and brain, and the lowest level in the liver in rats. However, the present results suggested that APEX/Ref-1 gene product can interact with AP-1 binding proteins in brain, especially in the hippocampal formation, to regulate some brain functions by redox-activation.
• Technical data
• Conjugation
  histidine
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  APEX1   APEx   Ref-1   Protein   His   tag  
• Gene symbol
  APEX1
• Short name
  Recombinant APEX1 / AP / APEx / Ref-1 Protein (His tag)
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
• Label
  AP
• Species
  Human, Humans
• Alternative name
  Rec. H. sapiens APEX1 / AP / APEx / Ref-1 Protein (histidine detection labelled)
• Alternative technique
  rec

Gene info

• Identity
  HGNC:587
• Gene
  APEX1
• Long gene name
  apurinic/apyrimidinic endodeoxyribonuclease 1
• Synonyms gene
  • APEX
• Synonyms gene name
  • APEX nuclease (multifunctional DNA repair enzyme)
  • APEX nuclease (multifunctional DNA repair enzyme) 1
• Synonyms
  • APE
  • REF1
  • HAP1
  • APX
  • APEN
  • REF-1
  • APE-1
• GenBank acession
  • X59764
• Locus
  14q11.2
• Discovery year
  1997-05-22
• Entrez gene record
  328
• RefSeq identity
  • NM_001641
• Classification
  • SET complex
  • Endoribonucleases
• VEGA ID
  OTTHUMG00000029544
• Locus Specific Databases
  • ALSOD, the Amyotrophic Lateral Sclerosis Online Genetic Database

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

Similar products