Recombinant Human Alkaline Phosphatase/ALPP/PLAP (C-6His)
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Catalog number
CB45-1000
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Price
Please ask
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Size
1 mg
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Description
Recombinant Human Alkaline Phosphatase is produced by our Mammalian expression system and the target gene encoding Ile23-Asp506 is expressed with a 6His tag at the C-terminus.
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Species reactivity
Human
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Origin
Human Cells
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Peptide sequence
IIPVEEENPDFWNREAAEALGAAKKLQPAQTAAKNLIIFLGDGMGVSTVTAARILKGQKKDKLGPEIPLAMDRFPYVALSKTYNVDKHVPDSGATATAYLCGVKGNFQTIGLSAAARFNQCNTTRGNEVISVMNRAKKAGKSVGVVTTTRVQHASPAGTYAHTVNRNWYSDADVPASARQEGCQDIATQLISNMDIDVILGGGRKYMFRMGTPDPEYPDDYSQGGTRLDGKNLVQEWLAKRQGARYVWNRTELMQASLDPSVTHLMGLFEPGDMKYEIHRDSTLDPSLMEMTEAALRLLSRNPRGFFLFVEGGRIDHGHHESRAYRALTETIMFDDAIERAGQLTSEEDTLSLVTADHSHVFSFGGYPLRGSSIFGLAPGKARDRKAYTVLLYGNGPGYVLKDGARPDVTESESGSPEYRQQSAVPLDEETHAGEDVAVFARGPQAHLVHGVQEQTFIAHVMAFAACLEPYTACDLAPPAGTTDVDHHHHHH
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Estimated molecular weight
53,7 kDa
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Protein purity
Greater than 95% as determined by reducing SDS-PAGE.
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Endotoxin level
Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.
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Shipping condition
Dry Ice/ice packs
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Package form
Supplied as a 0.2 µm filtered solution of PBS, pH 7.4 .
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Storage conditions
Store at below -20°C, stable for 6 months after receipt.Please minimize freeze-thaw cycles.
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Reconstitution conditions
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 µg/ml.Dissolve the lyophilized protein in ddH2O.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
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UniProt number
P05187
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
ALPP, ASPN, LNPEP, PLAA
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Short name
Recombinant Alkaline Phosphatase/ALPP/PLAP (C-6His)
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. novo advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Label
Alkaline Phosphatase
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Species
Human, Humans
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Alternative name
Human Alkaline Phosphatase/ALPP/PLAP(C-6His)
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Alternative technique
rec
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Gene info
Gene info
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Identity
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Gene
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Long gene name
asporin
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Synonyms gene name
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year
2001-03-21
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small leucine rich repeat proteoglycans
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VEGA ID
Gene info
Gene info
MeSH Data
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Name
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Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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