Recombinant Human ACVR2B / ActivinR-IIB Protein (Fc tag)

• Catalog number
  PKSH031743-100µg
• Price
  Please ask
• Size
  100µg

• Synonym
  Activin RIIB;ActR-IIB;ACTRIIB;HTX4
• Activity
  Measured by its ability to neutralize Activin-mediated inhibition on MPC11 cell proliferation. The ED50 for this effect is typically 0.02-0.1 µg/mL in the presence of 10 ng/mL recombinant Activin A.2. Measured by its binding ability in a functional ELISA. 3. Immobilized human ACVR2B (Cat:10229-H02H) at 10 μg/mL (100 μl/well) can bind biotinylated human INHBA-His (Cat:10429-H08H), The EC50 of biotinylated human INHBA-His (Cat:10429-H08H) is 0.112 μg/mL.4. Immobilized human ACVR2B (Cat:10229-H02H) at 10 μg/mL (100 μl/well) can bind biotinylated mouse INHBA-His (Cat:Cat:50659-M08H), The EC50 of biotinylated mouse INHBA-His (Cat:Cat:50659-M08H) is 0.161 μg/mL.
• Sequence
  Met 1-Thr 134
• Fusion tag
  C-Fc
• Accession
  NP_001097.2
• Expressed Host
  HEK293 Cells
• Shipping
  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
• Purity
  ＞97 % as determined by SDS-PAGE
• Endotoxin
  ＜1.0 EU per µg as determined by the LAL method
• Stability and Storage
  Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Mol Mass
  40.0 kDa
• AP Mol Mass
  60-65 kDa
• Formulation
  Lyophilized from sterile PBS, pH 7.4
• Background
  ACVR2A and ACVR2B are two activin type II receptors. ACVR2B is integral to the activin and myostatin signaling pathway. Ligands such as activin and myostatin bind to ACVR2A and ACVR2B. Myostatin, a negative regulator of skeletal muscle growth, is regarded as a potential therapeutic target and binds to ACVR2B effectively, and to a lesser extent, to ACVR2A. The structure of human ACVR2B kinase domain in complex with adenine establishes the conserved bilobal architecture consistent with all other catalytic kinase domains. Haplotype structure at the ACVR2B and follistatin loci may contribute to interindividual variation in skeletal muscle mass and strength. Defects in ACVR2B are a cause of left-right axis malformations.
• Technical data
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  ACVR2B   ActivinR-IIB   Protein   tag  
• Gene symbol
  ACVR2B, MYH4, ACVR2B-AS1
• Short name
  Recombinant ACVR2B / ActivinR-IIB Protein (Fc tag)
• Technique
  Recombinant, FC, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
• Label
  Fc Tag
• Species
  Human, Humans
• Alternative name
  Rec. H. sapiens ACVR2B / ActivinR-IIB Protein (fragment c detection labelled)
• Alternative technique
  rec

Gene info

• Identity
  HGNC:174
• Gene
  ACVR2B
• Long gene name
  activin A receptor type 2B
• Synonyms gene name
  • activin A receptor, type IIB
• Synonyms
  • ActR-IIB
• GenBank acession
  • X77533
• Locus
  3p22.2
• Discovery year
  1997-03-19
• Entrez gene record
  93
• Pubmed identfication
  • 8161782
  • 9621519
• RefSeq identity
  • NM_001106
• Classification
  • Type 2 receptor serine/threonine kinases
• VEGA ID
  OTTHUMG00000131291

Gene info

• Identity
  HGNC:7574
• Gene
  MYH4
• Long gene name
  myosin heavy chain 4
• Synonyms gene name
  • myosin, heavy polypeptide 4, skeletal muscle
• Synonyms
  • MYH2B
  • MyHC-2B
  • MyHC-IIb
• Locus
  17p13.1
• Discovery year
  1986-01-01
• Entrez gene record
  4622
• Pubmed identfication
  • 8518795
• RefSeq identity
  • NM_017533
• Classification
  • Myosin heavy chains, class II
• VEGA ID
  OTTHUMG00000130365

Gene info

• Identity
  HGNC:44161
• Gene
  ACVR2B-AS1
• Long gene name
  ACVR2B antisense RNA 1
• Synonyms gene name
  • ACVR2B antisense RNA 1 (non-protein coding)
• GenBank acession
  • AK025619
• Locus
  3p22.2
• Discovery year
  2012-06-30
• Entrez gene record
  100128640
• RefSeq identity
  • NR_028389
• Classification
  • Antisense RNAs
• VEGA ID
  OTTHUMG00000156031

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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