Recombinant EGFP

We send this reagant on

Blue Ice
The temperatureerature for storage is

-80ºC; avoid freeze/thaw
Technical file
SDS or safty data sheet

more (STA-201) GHS.pdf
Type of reagent

Cell Signaling and Protein Biology
Related techniques

Cell Signaling and Protein Biology, Reporter Assays, Cells and Reagents, Recombinant Fluorescent Proteins
Use of the reagent

Recombinant EGFP contains a 6x-His tag at the C-terminus. Provided as 5 vials of 100 µg at a concentration of 1 mg/mL.
Cellular use

Includes 6xHis-tag at C-terminus
Protocol information

Provided at 100 μg/vial at 1.0 mg/mL in 1X PBS
Living modified organisme in this reagent or LMO

Recombinant Protein purified from E. coli
Species compatible to

Humans
Use of this test kit or product

Genetics lab
Antigen

a specific protein sequence
Buffer

The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7,2
Molecular weight

in kilo Danlto or kDa
This reagent is purified

0.97
Conjugate

The Green Fluorescent Protein (eGFP) of Recombinant can be excited at 488 nanometers with a peak emission at 509 nanometers and is detected in the FL1 detector on the FACSCalibur or FACScan. The LSRII and all of the core's sorter flow cytometers can tell the difference between at the same time/together expressing eGFP and eYFP cells when the proper optical filters and experimental controls exist.
Source

Recombinants or rec. proteins
Group

recombinants

Short name

Recombinant
Technique

Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Cellular biology labs advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
Alternative name

Rec. EGFP
Alternative technique

rec

Name

Primary Cell Culture
Concept

Scope note: The initial culturing of cells derived directly from fresh TISSUES.
Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data