Recombinant E. coli Formamidopyrimidine-DNA Glycosylase/Fpg (C-6His)
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Catalog number
C142-50
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Price
Please ask
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Size
50 ug
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Description
Recombinant E.coli Fpg is produced by our E.coli expression system and the target gene encoding Met1-Lys269 is expressed with a 6His tag at the C-terminus.
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Species reactivity
E.coli
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Origin
Escherichia coli
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Peptide sequence
MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRLSDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKVLRYTDPRRFGAWLWTKELEGHNVLTHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMDNKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLKDFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQKLEHHHHHH
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Estimated molecular weight
31,4 kDa
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Protein purity
Greater than 95% as determined by reducing SDS-PAGE.
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Endotoxin level
Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.
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Shipping condition
Dry ice/ice packs
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Package form
Supplied as a 0.2 µm filtered solution of 20mM TrisHCl, 100mM NaCl, 1mM EDTA, 1mM DTT, 50% Glycerol, pH 7.8.
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Storage conditions
Store at below -20°C, stable for 6 months after receipt. Please minimize freeze-thaw cycles.
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Reconstitution conditions
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 µg/ml.Dissolve the lyophilized protein in ddH2O.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
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UniProt number
A7ZTI6
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Source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
PRKDC, HLA-DOA
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Short name
Recombinant Formamidopyrimidine-DNA Glycosylase/Fpg (C-6His)
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Technique
Recombinant, dna, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. novo advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Host
e. coli, Escherichia coli, Recombination of bioactive proteins and longer peptides in Escherichia Coli is done often with His tagging. novo supplies Rec. E. Coli affinity purified or tag purified antigens in 1 quantities or bulk volumes on request.
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Species
E. coli, E. coli
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Alternative name
E.coli Fpg(C-6His)
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Alternative technique
rec, escherichia
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Gene info
Gene info
MeSH Data
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Name
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Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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