Recombinant ATP Dependent DNA ligase I (LIG1)

Description

Recombinant Human ATP Dependent DNA ligase I expressed in: E.coli
Specifications

Host species: Homo sapiens (Human); Expression system: E.coli; Expression region: Gly484~Ile663; Purity: ≥ 95%; Endotoxin level:
Additional_information

Uniprot ID: P18858; Buffer composition: PBS, pH 7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.; Form: Freeze-dried powder; Predicted Molecular Mass (KD): 24.4kDa; Isoelectric Point: 7.8
Storage_and_shipping

Shipped on ice packs. Upon receipt keep at 2-8 °C for up to 1 month. For long term storage prepare working aliquots and keep frozen at -20 or -80 °C. Avoid repeated freezing and thawing.
Notes

Research Use Only.
Source

Recombinants or rec. proteins
Group

recombinants

Gene target

ATP Dependent DNA ligase LIG1
Gene symbol

LIG1, VPS9D1, LRIG1, PRKDC, NFATC2, HLA-DOA
Short name

Recombinant ATP Dependent DNA ligase I (LIG1)
Technique

Recombinant, dna, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Cloud Clone Corp advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
Alternative name

Rec. adenosine triphosphate Dependent Desoxyribonucleic acid ligase I (LIG1)
Alternative technique

rec

Identity

HGNC:6598
Gene

LIG1
Long gene name

DNA ligase 1
Synonyms gene name
- ligase I, DNA, ATP-dependent
Synonyms name
- DNA ligase I
Locus

19q13.33
Discovery year

1991-05-09
Entrez gene record

3978
Pubmed identfication
- 1733856
RefSeq identity
- NM_000234
Classification
- Nucleotide excision repair
- DNA ligases
VEGA ID

OTTHUMG00000183187
Locus Specific Databases
- LIG1base: Mutation registry for DNA ligase I deficiency
- LRG_78

Identity

HGNC:17360
Gene

LRIG1
Long gene name

leucine rich repeats and immunoglobulin like domains 1
Synonyms
- LIG-1
- DKFZP586O1624
- LIG1
Synonyms name
- ortholog of mouse integral membrane glycoprotein LIG-1
- leucine-rich repeat protein LRIG1
GenBank acession
- AB050468
Locus

3p14.1
Discovery year

2002-08-23
Entrez gene record

26018
Pubmed identfication
- 11414704
- 12234026
RefSeq identity
- NM_015541
Classification
- I-set domain containing
VEGA ID

OTTHUMG00000158727

Identity

HGNC:7776
Gene

NFATC2
Long gene name

nuclear factor of activated T cells 2
Synonyms gene name
- nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2
- nuclear factor of activated T-cells 2
Synonyms
- NF-ATP
- NFATp
- NFAT1
GenBank acession
- U43342
- U43341
Locus

20q13.2
Discovery year

1994-11-16
Entrez gene record

4773
Pubmed identfication
- 8202141
RefSeq identity
- NM_012340
Classification
- IPT domain containing
- Nuclear factors of activated T-cells
- MicroRNA protein coding host genes
VEGA ID

OTTHUMG00000032747

Name

Primary Cell Culture
Concept

Scope note: The initial culturing of cells derived directly from fresh TISSUES.
Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data