RE, N-terminal arginylation
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Catalog number
AS15 3050
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Price
Please ask
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Size
2x50 µg
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Available ordering format
Inquire
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Immunogen
KLH-conjugated synthetic peptide: H-REHKHANQHMSVC-NH2
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Affinity purified serum in PBS pH 7.4
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How to reconstitute
For reconstitution add 50 µl of sterile water to each tube
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Storage condition
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applications
Dot blot (Dot)
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Connected products
AS15 3050A | anti-RD | N-terminal arginylation, rabbit antibodies collection of antibodies to proteins involved in transcriptioncollection of antibodies to proteins involved in translationPlant protein extraction buffer Secondary antibodies
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Recommended dilutions for use
1 : 1000 (Dot)
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Molecular weight expected аpparent
see datasheet
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Verified reactivity
to be determined
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Possible reactivity
to be determined
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No reactivity
to be determined
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Supplementary information
MG132 or epoxomycin are recommended to use to inhibit proteasome and significantly increase signal from the arginylated proteins.
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References
To be added when available, antibody released in June 2016.
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Scientific context
Arginylation is a post-translational modification of an existing peptide chain by addition of an extra arginine. This modification changes primary sequence of protein as well as it's surface change. It is mediated by arginyltransferase ATE1. Arginylation plays an essential role in multiple physiological pathways, for example, in vivo arginylation constitutes a mechanism for degradation of preprocessed proteins or proteolytic fragments that bear Asp and Glu on their N-termini.
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Notes
Antibody is purified using substractive purification method.
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Protein number
Refer to NCBI
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TAIR number
Refer to NCBI
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Gene target
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Short name
RE, N-terminal arginylation
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Alternative name
RE, N-terminal arginylation
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