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Description
A Rabbit polyclonal antibody against Human, Mouse, Rat, Pig Thy1 Cell Surface Antigen (Thy1). This antibody is labeled with HRP.
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Specifications
Host: Rabbit; Species Reactivity: Human, Mouse, Rat, Pig; Clonality: polyclonal; Tested applications: WB, IHC; Concentration: 500ug/ml; Isotype: IgG; Conjugation: HRP
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Additional_information
Sequence of the immunogen: Thy1 (Gln20~Ile133); Buffer composition: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
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Storage_and_shipping
Upon receipt, store at -20°C or -80°C. Prepare working aliqotes prior to storage to avoid repeated freeze-thaw cycles.
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Notes
Research Use Only.
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Properties
If you buy Antibodies supplied by Cloud Clone Corp they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional description
Antigens are peptides or recombinant or native dependent on the production method. For cells, cell lines and tissues in culture till half confluency.
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Conjugation
HRP Conjugated
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus, Rattus norvegicus
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About
Pigs and the smaller guinea pigs are frequent used as models for humans. Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Group
Polyclonals and antibodies
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French translation
anticorps
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Gene target
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Gene symbol
THY1
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Short name
Thy1 Surface Antigen (Thy1) Polyclonal Antibody ( , Mouse, , ),
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Technique
Polyclonal, Antibody, antigen, Mouse, antibodies against human proteins, antibodies for, antigenes, mouses, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Host
mouse
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Label
HRP
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Species
Pig, Humans, Mouses, Pigs and Guinea pigs, Rats
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Alternative name
Thy-1 cellular surface antigen cellular Surface protein (Thy-1 cellular surface antigen) polyclonal (antibody to-) (H. sapiens, Mouse, Rat, swine), horseradish peroxidase
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Alternative technique
polyclonals, antibodies, antigenes, murine
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Alternative to gene target
Thy-1 cell surface antigen, CD90, THY1 and IDBG-74502 and ENSG00000154096 and 7070, GPI anchor binding, Cell surfaces, Thy1 and IDBG-155394 and ENSMUSG00000032011 and 21838, THY1 and IDBG-631960 and ENSBTAG00000019627 and 614712
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Tissue
cell
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