Rat IgG (Fc)-FITC Conjuagte (isotype control), purified

  • Catalog number
    20005-1-FC-F
  • Price
    Please ask
  • Size
    0.1 mg
  • Stock availability
    Available
  • Category
    Isotype Control
  • Antibody type
    N/A
  • Antibody host
    N/A
  • Antibody conjugate
    FITC
  • Technical datasheet
    Contact Gentaur to request the datasheet or ask our specialists for more information.
  • Notes
    The Rat IgG (Fc)-FITC Conjuagte (isotype control), purified is manufactured for Research Use Only or for diagnostics purposes.
  • Description
    Isotype or positive controls by peptides, antibodies and deactivated samples.
  • Properties
    This adi Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. adi FITC is highly sensitive to pH extremes. Immunoglobulin Isotype specific antibodies and controls are specific to the immunoglobulin heavy chains and immunoglobulin light chains. Mouse monoclonal antibodies from this clone have all the same affinity to the antigen. Also polyclonal antibodies are Isotype purified amd conjugated or if they are used as control non conjugated.
  • Conjugation
    Anti-FITC Antibody
  • About
    Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
  • Group
    isotypes
  • Latin name
    Rattus norvegicus
  • Gene target
  • Short name
    IgG (Fc)-FITC Conjuagte (isotype control), purified
  • Technique
    purified, Control, Isotype, FITC, IgG, FC, controls, Fluorescein, IgGs, Isotypes and controls, purifications
  • Host
    Rat
  • Isotype
    IgG, IgG
  • Label
    FITC
  • Species
    Rat, Rats
  • Alternative name
    Rat Immunoglobulin G (fragment c)-fluorecein Conjuagte (isoform reference), enriched
  • Alternative technique
    pure, control, controls, fluorescine, immunoglobulins
  • Tissue
    control
MeSH Data
  • Name
  • Concept
    Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
  • Tree numbers
    • E01.370.225.500.607.512.240
    • E01.370.225.750.551.512.240
    • E05.200.500.607.512.240
    • E05.200.750.551.512.240
    • E05.478.583.375
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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