Rat GRP78 ELISA Kit

  • Catalog number
    SKT-137-96
  • Price
    Please ask
  • Size
    96 well
  • Stock availability
    In Stock
  • Kit s description
    Colorimetric detection of GRP78 in rat lysates
  • Protein target
    GRP78
  • Scientific context
    GRP78 is a ubiquitously expressed, 78-kDa glucose-regulated protein, and is commonly referred to as an immunoglobin chain binding protein (BiP). The BiP proteins are categorized as stress response proteins because they play an important role in the proper folding and assembly of nascent protein and in the scavenging of misfolded proteins in the endoplasmic reticulum lumen. Translation of BiP is directed by an internal ribosomal entry site (IRES) in the 5’ nontranslated region of the BiP mRNA. BiP IRES activity increases when cells are heat stressed (1). GRP78 is also critical for maintenance of cell homeostasis and the prevention of apoptosis (2). Luo et al. have provided findings that suggest GRP78 is essential for embryonic cell growth and pluripotent cell survival (3). In terms of diseases, GRP78 has been shown to be a reliable biomarker of hypoglycemia, to serve a neuroprotective function in neurons exposed to glutamate and oxidative stress (4), and its protein levels are reduced in the brains of Alzheimer’s patients (5). Also, the induction of the GRP78 protein that results in severe glucose and oxygen deprivation could possible lead to drug resistance to anti­tumor drugs (6, 7).
  • Primary research area
    Cancer, Heat Shock, Cell Signaling, Trafficking, Chaperones, Organelle Markers
  • Category
    Assay ELISA Kits
  • Other name
    Grp78 ELISA Kit, Binding immunoglobulin protein ELISA Kit, BiP ELISA Kit, 78 kDa glucose-regulated protein ELISA Kit, heat shock 70 kDa protein 5 ELISA Kit
  • Brand name
    StressXpress®
  • Detection system
    Colorimetric Assay
  • Assay format
    Sandwich ELISA (Enzyme-linked Immunosorbent Assay)
  • Main intended usage
    ELISA kit used to quantitate GRP78 concentration in rat samples.
  • Brief protocol
    1. Prepare Standard and samples in Standard and Sample Diluent. 2. Add 100 µL of Standard or sample to appropriate wells. 3. Cover plate with Plate Sealer and incubate at 37°C for 2 hours. 4. Wash plate four times with 1X Wash Buffer. 5. Add 100 µL of Biotinylated Antibody Working Solution to each well. 6. Cover plate with Plate Sealer and incubate at at 37°C for 2 hours. 7. Wash plate four times with 1X Wash Buffer. 8. Add 100 µL of Streptavidin Poly HRP Working Solution to each well. 9. Cover plate with Plate Sealer and incubate at 37°C for 30 minutes. 10. Wash plate four times with 1X Wash Buffer. 11. Add 100 µL of TMB Substrate to each well. 12. Develop the plate in the dark at room temperature for 30 minutes. 13. Stop reaction by adding 100 µL of Stop Solution to each well. 14. Measure absorbance on a plate reader at 450 nm.
  • Kit contents
    Anti-Rat GRP78 Immunoassay Plate, Recombinant Rat GRP78 Standard , Standard and Sample Diluent, Anti-Rat Grp78 Biotinylated Antibody Concentrate, 10X Wash Buffer Concentrate, Anti-Rat GRP78 Biotinylated Antibody Diluent, Streptavidin Poly HRP Concentrate, Streptavidin Poly HRP Diluent, TMB Substrate, Stop Solution, Plate Cover
  • Assay precision
    Intra-Assay Precision: Three samples of known concentration were assayed thirty times on one plate. The intra-assay coefficient of variation of the Nitrotyrosine ELISA has been determined to be
  • Assay cross reactivity
    Rat
  • Samples to be used with this kit
    Tissue
  • Maximum samples to be used with this kit
    40 samples in duplicate
  • Assay duration
    30 minutes
  • Kit s sensitivity
    0,15 ng/ml
  • Assay detection limit
    0 - 50 ng/ml
  • Storage recommendations
    4°C
  • Shipping recommendations
    Blue Ice
  • Bibliography
    1. Cho S., et al. (2007). Mol Cell Biol 27(1): 368-83. 2. Yang Y., et al. (1998) J Biol Chem 273: 25552-25555. 3. Luo S., et al (2006) 26 (15): 5688-97. 4. Yu Z., et al. (1999) Exp Neurol. 15: 302-314. 5. Koomagi R., et al. (1999) Anticancer Res. 19:4333- 4336. 6. Laquerre S., et al. (1998) J. Virology 72: 4940-4949. 7. Dong D., et al. (2005) Cancer Res 65(13): 5785-91.
  • Release date
    1-Oct-2015
  • PubMed number
    Refer to PubMed
  • Tested applications
    No data available / currently testing
  • Tested reactivity
    No data available / currently testing
  • Resources available upon request
    Kit Booklet, MSDS
  • Product image legend
    Typical Standard Curve for the Rat GRP78 ELISA Kit (Enzyme-Linked Immunosorbent Assay) StressXpress® - SKT-137. Assay Type: Sandwich ELISA. Detection Method: Colorimetric Assay. Assay Range: 0 - 50 ng/ml. Graph of the Typical Standard Curve for the Rat GRP78 ELISA Kit StressXpress - SKT-137
  • Warnings
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    2
  • Net weight g
    500
  • Properties
    E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
  • Test
    ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
  • About
    Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
  • Latin name
    Rattus norvegicus
  • Gene target
    GRP78  
  • Gene symbol
    HSPA5
  • Short name
    GRP78 ELISA Kit
  • Technique
    ELISA kit, ELISA, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests.
  • Host
    Rat, antigen assay ELISA kit 1 Kit (96 Wells)
  • Species
    Rat, Rats
  • Alternative name
    Rat GRP78 Enzyme-linked immunosorbent assay reagent
  • Alternative technique
    elisas, kits
  • Alternative to gene target
    v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
Gene info
  • Identity
  • Gene
  • Long gene name
    heat shock protein family A (Hsp70) member 5
  • Synonyms gene
  • Synonyms gene name
    • heat shock 70kD protein 5 (glucose-regulated protein, 78kD)
    • heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
  • Synonyms
  • Synonyms name
  • Locus
  • Discovery year
    1991-07-26
  • Entrez gene record
  • Classification
    • Heat shock 70kDa proteins
  • VEGA ID
MeSH Data
  • Name
  • Concept
    Scope note: An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
  • Tree numbers
    • E05.478.566.350.170
    • E05.478.566.380.360
    • E05.478.583.400.170
    • E05.601.470.350.170
    • E05.601.470.380.360
  • Qualifiers
    ethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data
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