Rat Anti Mouse F4/80 Antibody-FITC

• Catalog number
  MOF4F(V100)
• Price
  Please ask
• Size
  0,1 mg

• Description
  Rat Anti Mouse F4/80 Antibody-FITC is a mouse monoclonal antibody, specific for [BM8] clones, this product was conjugated with FITC format and reported for used in flow cytometric analysis to react with F4/80 glycoproteins that expressed in a large part of tissue macrophages and in subsets of dendritic cells, F4/80's function is the induction of immunological tolerance, also used as a murine macrophages marker.
• Other rodent antibodies
• Clone
  BM8
• Conjugate
  FITC
• Gene Name
  F4/80
• Other Names of gene
  EMR1, Ly71
• Concentration
  500 µg/mL
• Application
  Flow Cytometry Method
• Immunogen
  Murine macrophages
• Formulation
  Aqueous buffered solution containing protein stabilizer, and ≤0.09% sodium azide (NaN3).
• Storage
  2-8°C, store in dark, do not freeze
• Expiration
  After 1 year
• Remark
  For Recherche Use Only.
• Additional description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. This is against a protein of mus musculus directed antibody.
• Properties
  If you buy Antibodies supplied by Immunostep they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Immunostep Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Immunostep FITC is highly sensitive to pH extremes.
• Conjugation
  Anti-FITC Antibody
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus, Rattus norvegicus
• About
  Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
• French translation
  anticorps

• Gene target
  F4/80  
• Short name
  Anti Mouse F4/80 Antibody-FITC
• Technique
  anti mouse, Antibody, Mouse, anti, FITC, antibody to, anti mouse antibodies, antibodies against human proteins, antibodies for, Fluorescein, mouses
• Host
  Rat
• Isotype
  Rat IgG2a
• Label
  FITC
• Species
  Mouse, Mouses, Rats
• Alternative name
  Rat F4/80 Mouse Monoclonal Antibody [BM8]-FITC
• Alternative technique
  antibodies, murine, fluorescine

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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