Rat SCUBE1(Signal Peptide, CUB and EGF-like Domain-containing Protein 1) EIA Kit

• Catalog number
  E-EL-R1103-96T
• Price
  Please ask
• Size
  96Test

• Detection Type
  Colormetric
• Target Species
  Rat
• Target Synonym
  signal peptide-CUB domain-EGF-related 1
• Test principle
  This EIA kit uses the Sandwich-EIA principle. The micro EIA plate provided in this kit has been pre-coated with an antibody specific to Rat SCUBE1. Standards or samples are added to the micro EIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat SCUBE1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat SCUBE1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat SCUBE1. You can calculate the concentration of Rat SCUBE1 in the samples by comparing the OD of the samples to the standard curve.
• Sensitivity
  0.38ng/mL
• Detection Range
  0.63~40ng/mL
• Target Name
  SCUBE1
• UNIProt ID
  F1M987
• Tested Sample Types
  Serum, Plasma, Cell supernatant
• Product Name
  Rat SCUBE1(Signal Peptide, CUB and EGF-like Domain-containing Protein 1) EIA Kit
• Type
  Sandwich
• Technical data
• Standard Curve RUL
• Description
  This ELISA test kit or EIA Enzyme immuno assay is an enzyme linked immuno assay supplied as coated 96 well plates with antigen or antibody and needs to be stored at +4°C. Peptides short amino acid chains or epitopes or blocking antagonists. The shortest peptides are dipeptides, consisting of 2 amino acids joined by a single peptide bond, followed by tripeptides, tetra peptides, ... till polypeptides that are long, continuous, and unbranched synthetic peptide chains. These biological oligomers and polymers can be Solid-phase peptide synthesis (SPPS), or in continue produced for custom peptide synthesis projects. The High-efficiency solid phase peptide synthesis (HE-SPPS) is give very low production costs.
• Properties
  MeSH E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay
• About
  Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
• Latin name
  Rattus norvegicus

• Gene target
  SCUBE1   Signal   Peptide   CUB   EGF-like   Domain   Protein   Kit  
• Gene symbol
  EGF, SCUBE1, SCUBE1-AS1, SCUBE1-AS2
• Short name
  SCUBE1(Signal Peptide, CUB EGF-like Domain- Protein 1) EIA Kit
• Technique
  peptide, EIA, eia, peptides
• Host
  Rat
• Species
  Rat, Rats
• Alternative name
  Rat SCUBE1(Signal short protein sequence, CUB and epidermal growth factor-like Domain-containing Protein 1) EIA reagent
• Alternative technique
  peptides, kits, elisas
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

Gene info

• Identity
  HGNC:3229
• Gene
  EGF
• Long gene name
  epidermal growth factor
• Synonyms gene name
  • epidermal growth factor (beta-urogastrone)
• Synonyms name
  • Pro-epidermal growth factor
• GenBank acession
  • X04571
• Locus
  4q25
• Discovery year
  2001-06-22
• Entrez gene record
  1950
• VEGA ID
  OTTHUMG00000132044

Gene info

• Identity
  HGNC:13441
• Gene
  SCUBE1
• Long gene name
  signal peptide, CUB domain and EGF like domain containing 1
• Synonyms gene name
  • signal peptide, CUB domain, EGF-like 1
• Locus
  22q13.2
• Discovery year
  2002-02-13
• Entrez gene record
  80274
• Pubmed identfication
  • 11087664
• RefSeq identity
  • NM_173050
• VEGA ID
  OTTHUMG00000150679

Gene info

• Identity
  HGNC:40635
• Gene
  SCUBE1-AS1
• Long gene name
  SCUBE1 antisense RNA 1
• Locus
  22q13.2
• Discovery year
  2020-03-19
• Entrez gene record
  101927447
• RefSeq identity
  • NR_134582
• Classification
  • Antisense RNAs

Gene info

• Identity
  HGNC:40633
• Gene
  SCUBE1-AS2
• Long gene name
  SCUBE1 antisense RNA 2
• Locus
  22q13.2
• Discovery year
  2020-03-19
• Entrez gene record
  105373051
• RefSeq identity
  • NR_134632
• Classification
  • Antisense RNAs

MeSH Data

• Name
  Immunoenzyme Techniques
• Concept
  Scope note: Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.
• Tree numbers
  • E05.478.566.350
  • E05.478.583.400
  • E05.601.470.350
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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