Rat CFB(Complement Factor B) EIA Kit

  • Catalog number
    E-EL-R0253-96T
  • Price
    Please ask
  • Size
    96Test
  • Detection Type
    Colormetric
  • Target Species
    Rat
  • Target Synonym
    AHUS4, BF, BFD, CFAB, FB, FBI12, GBG, H2-Bf, PBF2, Properdin
  • Test principle
    This EIA kit uses the Sandwich-EIA principle. The micro EIA plate provided in this kit has been pre-coated with an antibody specific to Rat CFB. Standards or samples are added to the micro EIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CFB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CFB, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat CFB. You can calculate the concentration of Rat CFB in the samples by comparing the OD of the samples to the standard curve.
  • Sensitivity
    1.88ng/mL
  • Detection Range
    3.13~200ng/mL
  • Target Name
    CFB
  • UNIProt ID
    G3V615
  • Tested Sample Types
    Serum, Plasma, Cell supernatant
  • Product Name
    Rat CFB(Complement Factor B) EIA Kit
  • Type
    Sandwich
  • Description
    This ELISA test kit or EIA Enzyme immuno assay is an enzyme linked immuno assay supplied as coated 96 well plates with antigen or antibody and needs to be stored at +4°C. Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.
  • Properties
    MeSH E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay
  • About
    Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
  • Latin name
    Rattus norvegicus
  • Gene target
    CFB   Complement   Factor   Kit  
  • Gene symbol
    CFB
  • Short name
    CFB(Complement Factor B) EIA Kit
  • Technique
    EIA, eia
  • Host
    Rat
  • Species
    Rat, Rats
  • Alternative name
    Rat Complement factor B Ba fragment; Uncharacterized protein; cDNA FLJ55673, highly similar to Complement factor B(Complement Factor B) EIA reagent
  • Alternative technique
    kits, elisas
  • Alternative to gene target
    v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
Gene info
  • Identity
  • Gene
    CFB
  • Long gene name
    complement factor B
  • Synonyms gene
  • Synonyms gene name
    • B-factor, properdin
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    1986-01-01
  • Entrez gene record
    629
  • RefSeq identity
  • Classification
    • Complement system activation components
    • Sushi domain containing
  • VEGA ID
  • Locus Specific Databases
MeSH Data
  • Name
  • Concept
    Scope note: Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.
  • Tree numbers
    • E05.478.566.350
    • E05.478.583.400
    • E05.601.470.350
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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