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Clone
23c
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Immunogen
Purified recombinant mouse TCP1 alpha construct encoding the C-terminal half of the 1.8 kb full-length Tcp 1b gene expressed in E. coli. Detects the COOH group.
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Antibody s full description
Rat Anti-Mouse TCP1 alpha Monoclonal IgG2c Antibody, Clone: 23c: PerCP
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Antibody s category
Monoclonal Antibodies
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Antibody s other name
p63 Antibody, Tcp-1 Antibody, TCP1 alpha Antibody, Ccta Antibody, TRic Antibody, Tp63 Antibody, 21454 Antibody, c-cpn Antibody, CCT Antibody, Tcp1 Antibody, ccpn Antibody, AI528772 Antibody, Cct1 Antibody, Tcp 1 Antibody, c cpn Antibody
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Raised in
Rat
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Antibody s target
TCP1 alpha
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Primary research fields
Cancer, Heat Shock, Cell Signaling, Trafficking
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Brandname
None
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Antibodies applications
WB, ICC/IF, IP
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Antibody s reactivity
Mouse, Rat, Sheep, Rabbit, Hamster, Dog, Bovine
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Antibody s dilutions
WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
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Purity
Protein G Purified
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Antibody buffer for storage
PBS pH7.4, 50% glycerol, 0.1% sodium azide
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Antibody s concentration
1 mg/ml
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Antibody s specificity
Detects ~60kDa. Also detects ~92kDa. The addition of an alanine (LDDA COOH) prevents binding in ELISA assays to immobilized synthetic peptide sequences. This antibody recognizes other proteins, most notably the p102B' COP subunit of Golgi coatomer. It does not react with human HSP60 protein.
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Storage recommendations
-20ºC
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Shipping recommendations
Blue Ice or 4ºC
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Antibody certificate of analysis
1 µg/ml of SMC-478 was sufficient for detection of TCP1 alpha in 20 µg of 3T3 cell lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary antibody.
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Antibody in cell
Cytoplasm
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Tissue specificity
See included datasheet or contact our support service
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Scientific context
T-complex polypeptide-1 (TCP1) is a ~60 kDa protein constitutively expressed in almost all eukaryotic cells, and is up-regulated during spermatogenesis. It is found in the cytosol as a subunit of a hetero-oligomeric chaperone that is known to be involved in the folding of actin and tubulin. The family of proteins termed chaperonins act to recognize and stabilize polypeptide intermediates during folding, assembly and disassembly, and share many characteristics with Heat Shock Protein 70 (HSP 70) including high abundance, induction by environmental stress, and ATPase activity. The chaperonin family includes the mitochondrial HSP60, Escherichia coli GroEL, the plastid Rubisco-subunit binding protein, and the archaebacterial protein TF55. The TCP1 sequence shows nearly 40% identity to TF55, but only minimal similarity to HSP60 and GroEL.
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Released date
16-Sep-2013
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Tested applications
To be tested
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Tested reactivity
To be tested
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NCBI number
NP_038714.2
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Gene number
21454
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Protein number
P11983
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Antibody s datasheet
Contact our support service
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Representative figure link
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Representative figure legend
Immunocytochemistry/Immunofluorescence analysis using Rat Anti-TCP1-alpha Monoclonal Antibody, Clone 23c (SMC-478). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rat Anti-TCP1-alpha Monoclonal Antibody (SMC-478) at 1:100 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rat (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Centrosome. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-TCP1-alpha Antibody. (C) Composite. Heat Shocked at 42°C for 1h. Western Blot analysis of Human A431 and HEK293 cell lysates showing detection of TCP1 alpha protein using Rat Anti-TCP1 alpha Monoclonal Antibody, Clone 23c (SMC-478). Primary Antibody: Rat Anti-TCP1 alpha Monoclonal Antibody (SMC-478) at 1:1000. Immunocytochemistry/Immunofluorescence analysis using Rat Anti-TCP1-alpha Monoclonal Antibody, Clone 23c (SMC-478). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rat Anti-TCP1-alpha Monoclonal Antibody (SMC-478) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rat (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Centrosome. Magnification: 20x. Heat Shocked at 42°C for 1h. Rat Anti-TCP1-alpha Antibody [23c] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked HeLa Cells (SMC-478) Rat Anti-TCP1 alpha Antibody [23c] used in Western Blot (WB) on Human A431 and HEK293 cell lysates (SMC-478) Rat Anti-TCP1-alpha Antibody [23c] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked HeLa Cells (SMC-478)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availability
In Stock
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Description
The Anti-Mouse TCP1 alpha Monoclonal IgG2c, Clone 23c is a α- or alpha protein sometimes glycoprotein present in blood. This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus, Rattus norvegicus
