Anti-Mouse CD45RC, FITC (Clone IBL-8) (rat IgG1)
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Catalog number
MBS520167
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Price
Please ask
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Size
0.3 mg
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Products_type
Antibody
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Products_short_name
[CD45RC]
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Products_name_syn
[CD45RC, FITC (Clone IBL-8) (rat IgG1); FITC Anti-Mouse CD45RC Monoclonal Antibody]
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Products_gene_name
[CD45RC]
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Clonality
Monoclonal
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Clone
[IBL-8]
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Reactivity
Mouse
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Specificity
CD45RC (Mouse CD45RC)
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Form
FITC (FITC conjugated Ig buffered in PBS, 0.02% NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml.)
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Concentration
Antibody Concentration: 0.1 mg/ml
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Storage_stability
Store at 4 degree C. For long term storage, aliquot and freeze unused portion at -20 degree C in volumes appropriate for single usage. Avoid freeze/thaw cycles
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Tested_application
Immunohistochemistry (IHC)
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus, Rattus norvegicus
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About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Gene target
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Gene symbol
SNORD114-8, RNU6-8, SNORD113-8, SNORD116-8, IGKV2OR2-8, MIR1302-8, PIRC35
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Short name
Anti-Mouse CD45RC, FITC (Clone IBL-8) ( IgG1)
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Technique
anti-, Mouse, anti, FITC, antibody to, antibodies, Fluorescein, mouses
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Host
Rat
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Isotype
Rat IgG1/k, IgG1
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Label
FITC
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Species
Rat, Mouses, Rats
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Alternative name
Antibody toMouse CD45RC, fluorecein (clonality IBL-8) (rat IgG1)
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Alternative technique
murine, antibodies, fluorescine
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Clone name
IBL-8
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Gene info
Gene info
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Identity
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Gene
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Long gene name
RNA, U6 small nuclear 8
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Synonyms
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Locus
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Discovery year
2008-06-10
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
Gene info
Gene info
Gene info
Gene info
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Identity
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Gene
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Long gene name
microRNA 1302-8
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Synonyms gene
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Synonyms
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Locus
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Discovery year
2008-11-03
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Entrez gene record
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RefSeq identity
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Classification
Gene info
MeSH Data
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Name
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Concept
Scope note:
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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