Anti-Mouse CD45, FITC (Clone IBL-5/25) (rat IgG1)
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Catalog numberGEN520147
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PricePlease ask
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Size100ug
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Also known asCD45
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Other namesCD45; N/A; CD45; N/A; N/A
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CategoryAntibodies
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SubcategoryMnoclonal antibodies
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Gene nameCD45
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Gene name synonimsN/A
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Other gene namesptprc; N/A; N/A; N/A
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ClonalityMonoclonal
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Immunoglobulin isotypeRat IgG1/k
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CloneIBL-5/25
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Host organismRat (Rattus norvegicus)
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Species reactivityMouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
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Specificity and cross reactivityCD45 (LCA) (Mouse CD45 (L-CA)); Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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Purification methodN/A
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Form AppearanceFITC (FITC conjugated Ig buffered in PBS, 0.02% NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml.)
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ConcentrationAntibody Concentration: 0.1 miligram per 1 mililiter
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Storage and shippingStore the antibody at +4 degrees Celsius. For long term storage, aliquot and freeze unused portion the antibody should be stored at -20 degrees Celsius. in volumes appropriate for single usage. Avoid freeze/thaw cycles.
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Tested applicationsImmunohistochemistry (IHC)
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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PropertiesThis MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes.
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ConjugationAnti-FITC Antibody
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus, Rattus norvegicus
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AboutRats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Gene target
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Gene symbolPTPRCAP, MIR1302-5, PIRC23, PIRC22, PIRC20, PTPRC, PIRC21, HTR6, IGHV2OR16-5, IGKV3OR2-5
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Short nameAnti-Mouse CD45, FITC (Clone IBL-5/25) ( IgG1)
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Techniqueanti-, Mouse, anti, FITC, antibody to, antibodies, Fluorescein, mouses
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Hostmouse
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IsotypeIgG1
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LabelFITC
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SpeciesRat, Mouses, Rats
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Alternative nameAnti-Anti-Mouse CD45, FITC (Clone IBL-5/25) (rat IgG1)
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Alternative techniquemurine, antibodies, fluorescine
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Clone nameIBL-5
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Gene info
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Identity
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Gene
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Long gene nameprotein tyrosine phosphatase receptor type C associated protein
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Synonyms
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Locus
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Discovery year1996-04-22
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Entrez gene record
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemicroRNA 1302-5
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Synonyms gene
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Synonyms
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Locus
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Discovery year2008-11-03
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR1302 family
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 23
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 22
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 20
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene nameprotein tyrosine phosphatase receptor type C
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Synonyms gene
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Synonyms
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GenBank acession
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Locus
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Discovery year1986-01-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Protein tyrosine phosphatases receptor type
- Fibronectin type III domain containing
- CD molecules
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 21
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene name5-hydroxytryptamine receptor 6
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Synonyms gene name
- 5-hydroxytryptamine (serotonin) receptor 6
- 5-hydroxytryptamine (serotonin) receptor 6, G protein-coupled
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Synonyms
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GenBank acession
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Locus
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Discovery year1997-12-11
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- 5-hydroxytryptamine receptors, G protein-coupled
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable 2/OR16-5 (non-functional)
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Synonyms gene name
- immunoglobulin heavy variable 2/OR16-5
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-17
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Entrez gene record
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Classification
- Immunoglobulin heavy (IGH) orphons
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin kappa variable 3/OR2-5 (pseudogene)
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Synonyms gene name
- immunoglobulin kappa variable 3/OR2-5
- immunoglobulin kappa variable 3/OR2-5 pseudogene
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-18
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Entrez gene record
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Classification
- Immunoglobulin kappa (IGK) orphons
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data