Rat Anti Mouse CD25 Antibody-FITC

  • Catalog number
    MO25F(V25)
  • Price
    Please ask
  • Size
    0,025 mg
  • Description
    Rat Anti Mouse CD25 Antibody-FITC is a mouse monoclonal antibody, specific for [PC61] clones, this product was conjugated with FITC format and reported for use in flow cytometric analysis to identify CD25 glycoproteins in thymocyte subset, pre-B cells, activated T and B cells, also in regulatory T cells, CD25 acts as a IL-2 receptor, also plays a role in signal transduction.
  • Clone
    PC61
  • Conjugate
    FITC
  • Gene Name
    CD25
  • Other Names of gene
    IL-2Rα, Ly-43, p55, Tac
  • Concentration
    500 µg/mL
  • Application
    Flow Cytometry Method
  • Immunogen
    IL-2-dependent cytolytic mouse T-cell clone B6.1
  • Formulation
    Aqueous buffered solution containing protein stabilizer, and ≤0.09% sodium azide (NaN3).
  • Storage
    2-8°C, store in dark, do not freeze
  • Expiration
    After 1 year
  • Remark
    For Recherche Use Only.
  • Additional description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. This is against a protein of mus musculus directed antibody.
  • Properties
    If you buy Antibodies supplied by Immunostep they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Immunostep Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Immunostep FITC is highly sensitive to pH extremes.
  • Conjugation
    Anti-FITC Antibody
  • Test
    Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Latin name
    Mus musculus, Rattus norvegicus
  • About
    Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
  • French translation
    anticorps
  • Gene target
    CD25  
  • Gene symbol
    ISG20, IL2RA
  • Short name
    Anti Mouse CD25 Antibody-FITC
  • Technique
    anti mouse, Antibody, Mouse, anti, FITC, antibody to, anti mouse antibodies, antibodies against human proteins, antibodies for, Fluorescein, mouses
  • Host
    Rat
  • Isotype
    Rat IgG1
  • Label
    FITC
  • Species
    Mouse, Mouses, Rats
  • Alternative name
    Rat CD25 Mouse Monoclonal Antibody [PC61]-FITC
  • Alternative technique
    antibodies, murine, fluorescine
Gene info
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
  • Tree numbers
    • E01.370.225.500.386
    • E05.196.712.516.600.240
    • E05.200.500.386
    • E05.242.386
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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