Rat ALCAM PicoKine ELISA Kit

  • Catalog number
    EK1572
  • Price
    Please ask
  • Size
    1x96 well plate
  • ELISA s application
    For quantitative measurement of rat ALCAM in cell culture supernatant, cell lysate, tissue homogenates, serum and plasma heparin, EDTA .
  • Gene tested
    rat ALCAM
  • ELISA standard curve
    Concentration(pg/ml) + 0 + 78 + 156 + 312 + 625 + 1250 + 2500 + 5000 O.D. + 0.004 + 0.064 + 0.118 + 0.215 + 0.450 + 0.798 + 1.578 + 2.317
  • ELISA kit detection limits
    78pg/ml to 5000pg/ml
  • ELISA s sensitivity
    < 10pg/ml
  • Target of this ELISA
    Natural and recombinant rat ALCAM
  • Cross reaction
    the ELISA does not cross react with any other proteins except from closely related species
  • ELISA s precision parameters
    intra assay precisions and inter assay precisions samples;1;2;3;1;2;3 n;16;16;16;24;24;24 mean (pg/ml);189;722;2741;201;830;2905 ELISA standard deviations 9.26;37.54;158.98;11.86;50.63;203.35 CV(%);4.9;5.2;5.8;5.9;6.1;7.0
  • ELISA kit description
    GENTAUR's rat ALCAM ELISA test is a ready to use ELISA kit. We use a monoclonal from mouse specific for ALCAM has been precoated onto 96-well plates. Standards (Expression system for standard: NSO, Immunogen sequence: W28-K527) and your samples are measured with a biotin conjugated polyclonal from goat specific for ALCAM will than be washed with PBS. The colour reaction with TMB on HRP will colour the well from blue to yellow with the stop solution. The more rat ALCAM in your sample the higher the ELISA signal will be compared to this elisa's standard curve.
  • Detector and capure antibodies
    a monoclonal antibodies from mouse;a biotinylated polyclonal antibodies from goat
  • The ELISA contains
    Catalog number;Description;Quantity -;96-well plate precoated with anti- rat ALCAM antibodies;1 ST0000-10;Lyophilized recombinant rat ALCAM standard;10ng/tube×2 AR1107;Biotinylated anti- rat ALCAM antibodies;130μl(dilutions 1:100) AR1103;Avidin-Biotin-Peroxidase Complex (ABC);130μl(dilutions 1:100) AR1106-1;Sample diluent buffers;30ml AR1106-2;antibodies diluent buffers;12ml AR1106-3;ABC diluent buffers;12ml AR1104;TMB color developing agents;10ml AR1105;TMB stop solution;10ml -;Adhesive cover;4
  • Anti coagulation
    heparin or EDTA
  • How the elisa works
    Sample the amount of 100ul/well of the standard solution at 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 313pg/ml, 156pg/ml, 78pg/mll rat ALCAM standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffers into the control well (Zero well). Add 0.1ml of each properly diluted sample of rat cell culture supernatant, serum or plasma (heparin, EDTA) to the ELISA plate. Keep in mind that each rat ALCAM standard solution and each sample be measured in duplicate.
  • Minutes of TMB incubation
    25-30
  • More gene info
    CD166 antigen is a 100-105 kD typeI transmembrane glycoprotein that is a member of the immunoglobulin superfamily of proteins. In humans it is encoded by the ALCAM gene. The human gene was mapped to chromosome 3q13.1-q13.2 by fluorescence in situ hybridization of cDNA probes to metaphase chromosomes. It is also called CD166 (cluster of differentiation 166), MEMD, SC-1/DM-GRASP/BEN in the chicken, and KG-CAM in the rat. This gene is expressed on activated T cells, activated monocytes, epithelial cells, fibroblasts, neurons, melanoma cells, and also in sweat and sebaceous glands. CD166 plays an important role in mediating adhesion interactions between thymic epithelial cells and CD6+ cells during intrathymic T cell development. Recently, CD166 has also been used as a potential cancer stem cell marker.
  • Antigen
    NSO, W28-K527
  • Keep at
    Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
  • Uniprot
    CD166 antigen
  • Gene name
    activated leukocyte cell adhesion molecule
  • Alternative
    ALCAM protein ; CD166 antigen ; Protein MEMD ; Q13740
  • Gen protein identity
    O35112
  • Entrez nr
    79559
  • Properties
    E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
  • Test
    ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
  • About
    Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
  • Latin name
    Rattus norvegicus
  • Gene target
    ALCAM   PicoKine  
  • Gene symbol
    ALCAM
  • Short name
    ALCAM PicoKine ELISA Kit
  • Technique
    ELISA kit, ELISA, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests.
  • Host
    Rat, antigen assay ELISA kit 1 Kit (96 Wells)
  • Species
    Rat, Rats
  • Alternative name
    Rat activated leukocyte cellular adhesion molecule PicoKine Enzyme-linked immunosorbent assay reagent
  • Alternative technique
    elisas, kits
  • Alternative to gene target
    v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
Gene info
  • Identity
  • Gene
  • Long gene name
    activated leukocyte cell adhesion molecule
  • Synonyms gene name
    • activated leucocyte cell adhesion molecule
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    1997-01-17
  • Entrez gene record
    214
  • Pubmed identfication
  • RefSeq identity
  • Classification
    • Ig-like cell adhesion molecule family
    • C2-set domain containing
    • V-set domain containing
    • CD molecules
  • VEGA ID
MeSH Data
  • Name
  • Concept
    Scope note: An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
  • Tree numbers
    • E05.478.566.350.170
    • E05.478.566.380.360
    • E05.478.583.400.170
    • E05.601.470.350.170
    • E05.601.470.380.360
  • Qualifiers
    ethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data
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