RASGAP Antibody / RASA1
-
Catalog numberR32007
-
PricePlease ask
-
Size0.1mg
-
-
CategoryAntibody
-
Concentration0.5mg/ml if reconstituted with 0.2ml sterile DI water
-
FormAntigen affinity purified
-
ConjugationUnconjugated
-
ClonePolyclonal antibody
-
Recognised antigenRASGAP / RASA1
-
Host animalRabbit (Oryctolagus cuniculus)
-
ClonalityPolyclonal (rabbit origin)
-
Species reactivityHuman (Homo sapiens) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
-
Tested applicationsWB
-
Recommended dilutionsWestern blot: 0.1-0.5ug/ml
-
NotesOptimal dilution of the RASGAP antibody should be determined by the researcher.
-
Intented useThis RASGAP antibodyis to be used only for research purposes and not for diagnostics..
-
UniprotP20936
-
PurityAntigen affinity
-
DescriptionRASA1, also known as RAS p21 protein activator 1 or RASGAP, is a 120-kDa cytosolic human protein. The protein encoded by this gene is located in the cytoplasm and is part of the GAP1 family of GTPase-activating proteins. The gene product stimulates the GTPase activity of normal RAS p21 but not its oncogenic counterpart. Acting as a suppressor of RAS function, the protein enhances the weak intrinsic GTPase activity of RAS proteins resulting in the inactive GDP-bound form of RAS, thereby allowing control of cellular proliferation and differentiation. Mutations leading to changes in the binding sites of either protein are associated with basal cell carcinomas. Mutations also have been associated with hereditary capillary malformations (CM) with or without arteriovenous malformations (AVM) and Parkes Weber syndrome. Alternative splicing results in two isoforms where the shorter isoform, lacking the N-terminal hydrophobic region but retaining the same activity, appears to be abundantly expressed in placental but not adult tissues.
-
ImmunogenAmino acids AQQHVLKKLLAITELLQQKQNQYTKTNDVR of human RASA1 were used as the immunogen for the RASGAP antibody.
-
StorageAfter reconstitution, the RASGAP antibody may be kept for up to one month refrigerated at +4 degrees C.For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
-
PropertiesIf you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
-
French translationanticorps
-
Gene target
-
Gene symbolRASA1
-
Short nameAnti-RASGAP / RASA1
-
TechniqueAntibody, antibodies against human proteins, antibodies for
-
IsotypeRabbit IgG
-
Alternative nameAntibodies to RASGAP / RASA1
-
Alternative techniqueantibodies
-
Alternative to gene targetRAS p21 protein activator (GTPase activating protein) 1, CM-AVM and CMAVM and GAP and p120GAP and p120RASGAP and PKWS and RASA and RASGAP, RASA1 and IDBG-32785 and ENSG00000145715 and 5921, GTPase binding, Plasma membranes, Rasa1 and IDBG-170464 and ENSMUSG00000021549 and 218397, BT.102247 and IDBG-646529 and ENSBTAG00000009565 and 282032
-
Gene info
-
Identity
-
Gene
-
Long gene nameRAS p21 protein activator 1
-
Synonyms gene
-
Synonyms gene name
- RAS p21 protein activator (GTPase activating protein) 1
-
Synonyms
-
Synonyms name
-
Locus
-
Discovery year1989-06-30
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- SH2 domain containing
- C2 and RasGAP domain containing
- Pleckstrin homology domain containing
-
VEGA ID
-
Locus Specific Databases
MeSH Data
-
Name
-
ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data