RASA1 (Tyr460) Antibody, ALEXA FLUOR 488

• Catalog number
  bs-13281R-A488
• Price
  Please ask
• Size
  100ul

• Long name
  RASA1 (Tyr460) Polyclonal Antibody, ALEXA FLUOR 488 Conjugated
• Also known as
  Anti-RASA1 Tyr460 PAb ALEXA FLUOR 488
• Category
  Conjugated Primary Antibodies
• Conjugated with
  ALEXA FLUOR® 488
• Host Organism
  Rabbit (Oryctolagus cuniculus)
• Target Antigen
  RASA1 Tyr460
• Specificity
  This is a highly specific antibody against RASA1 Tyr460.
• Modification
  Phosphorylation
• Modification Site
  Tyr460
• Clonality
  Polyclonal
• Clone
  Polyclonal antibody
• Concentration
  1ug per 1ul
• Source
  KLH conjugated synthetic phosphopeptide derived from human GAP around the phosphorylation site of Tyr460
• Gene ID Number
  5921
• Tested applications
  IF(IHC-P)
• Recommended dilutions
  IF(IHC-P)(1:50-200)
• Crossreactivity
  Human, Mouse, Rat
• Cross reactive species details
  Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
• Background of the antigen
  The mammalian c-H-, c-K- and N-Ras proto-oncogenes encode ubiquitously expressed proteins (1,2). p21Ras can exist in either a physiologically quiescent GDP-binding state or a GTP-binding signal-emitting state (3). Oncogenic p21Ras proteins are trapped in the excited signal-emitting state because the mechanism normally employed to delimit their excitation period, hydrolysis of their bound GTP to GDP, is impaired as a result of specific mutations (3). Interaction of p21Ras with GTPase activating protein (GAP) can increase hydrolysis of p21Ras-bound GTP by as much as 1000-fold (4,5). The product of the neurofibromatosis type 1 gene (NF1) has also been shown to exhibit p21Ras GAP activity (6,7), and proteins that stimulate the GTPase activity of three other low molecular weight GTPases, including Rho, Rab 3A and Rap 1, have also been described (8,9).
• Purification
  Purified by Protein A.
• Storage conditions
  Store this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.
• Excitation emission
  499nm/519nm
• Synonyms
  GAP phospho Y460; p-GAP phospho Y460; Ras GAP; CM AVM; CMAVM; DKFZp434N071; GAP; GTPase activating protein; GTPase-activating protein; OTTHUMP00000222390; OTTHUMP00000222391; OTTHUMP00000222392; OTTHUMP00000222393; p120GAP; p120RASGAP; PKWS; Ras GTPase-activating protein 1; RAS p21 protein activator GTPase activating protein 1; Ras p21 protein activator; RASA; RASA1; RASA1_HUMAN; RasGAP; Triphosphatase activating protein.
• Properties
  For facs or microscopy Alexa 1 conjugate. Alexa Fluor 488 has the same range to that of fluorescein isothiocyanate (FITC), yet the Anti-RASA1 Tyr460 has a very high photo stability. As a result of this photo stability, it has turned into an antibody for fluorescent microscopy and FACS FLOW cytometry. It is distinguished in the FL1 of a FACS-Calibur or FACScan. Also Alexa Fluor 488 is pH stable. If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• Conjugation
  Alexa Fluor
• French translation
  anticorps

• Gene target
  RASA1   Tyr460  
• Gene symbol
  RASA1
• Short name
  Anti-RASA1 Tyr460
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Immunoglobulin G (IgG)
• Label
  ALEXA FLUOR 488
• Alternative name
  RASA1 (Tyr460) Polyclonal Antibody, ALEXA FLUOR 488
• Alternative technique
  antibodies
• Alternative to gene target
  RAS p21 protein activator (GTPase activating protein) 1, CM-AVM and CMAVM and GAP and p120GAP and p120RASGAP and PKWS and RASA and RASGAP, RASA1 and IDBG-32785 and ENSG00000145715 and 5921, GTPase binding, Plasma membranes, Rasa1 and IDBG-170464 and ENSMUSG00000021549 and 218397, BT.102247 and IDBG-646529 and ENSBTAG00000009565 and 282032

Gene info

• Identity
  HGNC:9871
• Gene
  RASA1
• Long gene name
  RAS p21 protein activator 1
• Synonyms gene
  • RASA
• Synonyms gene name
  • RAS p21 protein activator (GTPase activating protein) 1
• Synonyms
  • GAP
  • CM-AVM
  • p120GAP
  • p120RASGAP
  • p120
• Synonyms name
  • capillary malformation-arteriovenous malformation
  • p120 RAS GTPase activating protein
• Locus
  5q14.3
• Discovery year
  1989-06-30
• Entrez gene record
  5921
• Pubmed identfication
  • 15917201
• RefSeq identity
  • NM_002890
• Classification
  • SH2 domain containing
  • C2 and RasGAP domain containing
  • Pleckstrin homology domain containing
• VEGA ID
  OTTHUMG00000162605
• Locus Specific Databases
  • SH2base: Database for pathogenic SH2 domain mutations

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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